Evaluation of persistence of Adoxophyes orana granulovirus and Cydia pomonella granulovirus in populations of their hosts by molecular methods

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F08%3A00000893" target="_blank" >RIV/00027006:_____/08:00000893 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Evaluation of persistence of Adoxophyes orana granulovirus and Cydia pomonella granulovirus in populations of their hosts by molecular methods

Popis výsledku v původním jazyce

Polymerase chain reaction (PCR) based techniques were developed for the detection of AdorGV and CpGV in survived larvae collected in virus treated plots. Primers were based on the granulin gene sequence. Results indicate a strong persistence of AdorGV insurviving larvae after direct treatment causing high mortality of larvae in the next generations. The population density was reduced below the damage threshold within two years after the AdorGV virus treatment. CpGV treatment caused high mortality of C.pomonella larvae in the year of virus treatment. CpGV was found in as many as 15% of the surviving larvae 1 year after treatment in one location. The virus was not detected in CpGV-treated orchards 2 years after treatment. In contrast to persistence ofAdorGV in A. orana population, poor persistence of CpGV in surviving C. pomonella individual was recorded probably resulting in a slow spread in natural host populations.

Název v anglickém jazyce

Evaluation of persistence of Adoxophyes orana granulovirus and Cydia pomonella granulovirus in populations of their hosts by molecular methods

Popis výsledku anglicky

Polymerase chain reaction (PCR) based techniques were developed for the detection of AdorGV and CpGV in survived larvae collected in virus treated plots. Primers were based on the granulin gene sequence. Results indicate a strong persistence of AdorGV insurviving larvae after direct treatment causing high mortality of larvae in the next generations. The population density was reduced below the damage threshold within two years after the AdorGV virus treatment. CpGV treatment caused high mortality of C.pomonella larvae in the year of virus treatment. CpGV was found in as many as 15% of the surviving larvae 1 year after treatment in one location. The virus was not detected in CpGV-treated orchards 2 years after treatment. In contrast to persistence ofAdorGV in A. orana population, poor persistence of CpGV in surviving C. pomonella individual was recorded probably resulting in a slow spread in natural host populations.

Druh

D - Stať ve sborníku

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

IOBC/wprs Bulletin 2008

ISBN

978-92-9067-205-0

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

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Název nakladatele

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Místo vydání

N

Místo konání akce

Alés, France

Datum konání akce

1. 1. 2007

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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