Porovnání tří technik pro detekci viru svinutky révy 1.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F05%3A1282" target="_blank" >RIV/00027006:_____/05:1282 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of three techniques for the detection of Grapevine leafroll-associated virus 1.

Popis výsledku v původním jazyce

Thirty seven plants of grapevine from the Research Station of Viticulture, Karlštejn was examined for the presence of leafroll viruses. Grapevine leafroll-associated virus 1 (GLRaV-1) was detected in the grapevines plants tested using double-antibody sandwich ELISA (DAS-ELISA), RT-PCR and molecular hybridization with non-radioactive RNA probes. Both molecular methods were based on a detection of the GLRaV-1 heat-shock protein 70 (HSP70) gene and showed a higher sensitivity in the detection of GLRaV-1 compared to DAS-ELISA. RNA probes are considered more suitable for the GLRaV-1 detection, as their application can overcome potential minor sequence variability, which may cause the detection by RT-PCR less reliable, especially when the variability occursin the genome region targeted by RT-PCR primers. Based on additional DAS-ELISA, other viruses were detected, often in a mix infection: Grapevine leafroll-associated virus 3 (GLRaV-3), Grapevine virus A (GVA) and Grapevine fleck virus (GFk

Název v anglickém jazyce

Comparison of three techniques for the detection of Grapevine leafroll-associated virus 1.

Popis výsledku anglicky

Thirty seven plants of grapevine from the Research Station of Viticulture, Karlštejn was examined for the presence of leafroll viruses. Grapevine leafroll-associated virus 1 (GLRaV-1) was detected in the grapevines plants tested using double-antibody sandwich ELISA (DAS-ELISA), RT-PCR and molecular hybridization with non-radioactive RNA probes. Both molecular methods were based on a detection of the GLRaV-1 heat-shock protein 70 (HSP70) gene and showed a higher sensitivity in the detection of GLRaV-1 compared to DAS-ELISA. RNA probes are considered more suitable for the GLRaV-1 detection, as their application can overcome potential minor sequence variability, which may cause the detection by RT-PCR less reliable, especially when the variability occursin the genome region targeted by RT-PCR primers. Based on additional DAS-ELISA, other viruses were detected, often in a mix infection: Grapevine leafroll-associated virus 3 (GLRaV-3), Grapevine virus A (GVA) and Grapevine fleck virus (GFk

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

—

Projekt

<a href="/cs/project/GP522%2F01%2FD131" target="_blank" >GP522/01/D131: Charakterizace closterovirů spojených se svinutkou révy vinné na území České republiky.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Virologica

ISSN

0001-723X

e-ISSN

—

Svazek periodika

49

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

7

Strana od-do

37-43

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—