Analysis of multiple virus-infected grapevine plant reveals persistence but uneven virus distribution

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F09%3A00000709" target="_blank" >RIV/00027006:_____/09:00000709 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of multiple virus-infected grapevine plant reveals persistence but uneven virus distribution

Popis výsledku v původním jazyce

The LN33 grapevine plant was artificially infected with Grapevine leafroll-associated virus-1 (GLRaV-1), Grapevine virus A (GVA), Grapevine virus B (GVB), Rupestris stem pitting-associated virus (RSPaV), and an unclassified tymovirus. Although infected with GVB, corky bark symptoms never appeared, and this virus had no destructive effects on the LN33 grapevines under our experimental conditions. Dormant canes, sampled before the beginning of the vegetation period, were used as suitable targets to detectmentioned viruses using both the ELISA and RT-PCR method. For GLRaV-1, the ELISA- based diagnosis unexpectedly appeared more effective than RT-PCR; probably due to the insufficient specificity of the primers used, not reflecting the actual genetic variability of the virus. Distribution of viruses in long-term infected grapevine plants showed different degrees of irregularity, depending on the individual viruses.

Název v anglickém jazyce

Analysis of multiple virus-infected grapevine plant reveals persistence but uneven virus distribution

Popis výsledku anglicky

The LN33 grapevine plant was artificially infected with Grapevine leafroll-associated virus-1 (GLRaV-1), Grapevine virus A (GVA), Grapevine virus B (GVB), Rupestris stem pitting-associated virus (RSPaV), and an unclassified tymovirus. Although infected with GVB, corky bark symptoms never appeared, and this virus had no destructive effects on the LN33 grapevines under our experimental conditions. Dormant canes, sampled before the beginning of the vegetation period, were used as suitable targets to detectmentioned viruses using both the ELISA and RT-PCR method. For GLRaV-1, the ELISA- based diagnosis unexpectedly appeared more effective than RT-PCR; probably due to the insufficient specificity of the primers used, not reflecting the actual genetic variability of the virus. Distribution of viruses in long-term infected grapevine plants showed different degrees of irregularity, depending on the individual viruses.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/QG50083" target="_blank" >QG50083: Využití molekulární hybridizace pro detekci závažných virových patogenů révy vinné</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Virologica

ISSN

0001-723X

e-ISSN

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Svazek periodika

53

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

5

Strana od-do

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Kód UT WoS článku

000272972600010

EID výsledku v databázi Scopus

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