Možnosti rozlišení půdních bakterií FT-IR spektroskopií

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F05%3A1509" target="_blank" >RIV/00027006:_____/05:1509 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

An attempt to characterize some soil and health relevant bacteria by FT-IR spectroscopy

Popis výsledku v původním jazyce

There has been an increasing concern about health relevant bacteria that could be spread in soil and other environments by application of organic wastes or due to deliberate releases. Therefore a rapid and reliable identification of such potential pathogenic agents and their differentiation from common soil bacteria is required. We attempted to use FT-IR spectroscopy for this purpose. Soil bacteria Arthrobacter oxydans and Azotobacter chroococcum, and facultatively pathogenic bacteria Enterobacter agglomerans and Escherichia coli, all of them delivered well distinguished IR spectra in the following waverange: 3300 cm-1 mainly for nucleic acids structural bases; 3000-2800 cm-1 for cell membrane fatty acids; 1800-1500 cm-1 for saturated esters and cell proteins; 1500 cm-1 for proteins, lipids and phosphoesters; 1200-800 cm-1 for glycopeptides and phosphate groups of nucleic acids constituents. However, if cell mass was harvested in different phases of bacterial growth or bacteria were cu

Název v anglickém jazyce

An attempt to characterize some soil and health relevant bacteria by FT-IR spectroscopy

Popis výsledku anglicky

There has been an increasing concern about health relevant bacteria that could be spread in soil and other environments by application of organic wastes or due to deliberate releases. Therefore a rapid and reliable identification of such potential pathogenic agents and their differentiation from common soil bacteria is required. We attempted to use FT-IR spectroscopy for this purpose. Soil bacteria Arthrobacter oxydans and Azotobacter chroococcum, and facultatively pathogenic bacteria Enterobacter agglomerans and Escherichia coli, all of them delivered well distinguished IR spectra in the following waverange: 3300 cm-1 mainly for nucleic acids structural bases; 3000-2800 cm-1 for cell membrane fatty acids; 1800-1500 cm-1 for saturated esters and cell proteins; 1500 cm-1 for proteins, lipids and phosphoesters; 1200-800 cm-1 for glycopeptides and phosphate groups of nucleic acids constituents. However, if cell mass was harvested in different phases of bacterial growth or bacteria were cu

Druh

C - Kapitola v odborné knize

CEP obor

DF - Pedologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Emerging Biological Threat

ISBN

1-58603-555-X

Počet stran výsledku

9

Strana od-do

148-156

Počet stran knihy

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Název nakladatele

IOS press,

Místo vydání

Amsterdam

Kód UT WoS kapitoly

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