Partial characters of Plum pox virus ´Greek´ and its genetic variability in transgenic Prunus domestica L.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F06%3A1486" target="_blank" >RIV/00027006:_____/06:1486 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Partial characters of Plum pox virus ´Greek´ and its genetic variability in transgenic Prunus domestica L.

Popis výsledku v původním jazyce

The PPV genome region spanning C-terminal of NIb gene, coat protein (CP) and 3'-non translated region (NCR) gene of a challenging PPV isolate Greek interacting with in transgenic plum has characterized. Different typing assays including ELISA, RT-PCR, RLFP and sequencing (Cter-NIb, CP and 3'NCR region) reveals that challenging PPV-Greek belongs to a typical M subgroup. High sequence (both nucleotide 'nt' and amino acid 'aa') homology were found with M type isolates SK68 (nt 99.0% and aa 100%) or PS (nt98.1% and aa 99.3%) and with recombinant isolate BOR 3 (nt 96.0% and aa 97.4%). The PPV-Greek detected in transgenic plum, resistance clone C-5 or susceptible clone C-6 has shown a few mutations, at the position nt 8441 of Cter-NIb and nt 9179 of CP genein C-6 and at the position nt 9068 in C-5. The mutation in CP gene seems to drive from transgenic transcript, suggest the evidence of template switching between CP cistron of challenging virus and homologous transgenic transcript. These

Název v anglickém jazyce

Partial characters of Plum pox virus ´Greek´ and its genetic variability in transgenic Prunus domestica L.

Popis výsledku anglicky

The PPV genome region spanning C-terminal of NIb gene, coat protein (CP) and 3'-non translated region (NCR) gene of a challenging PPV isolate Greek interacting with in transgenic plum has characterized. Different typing assays including ELISA, RT-PCR, RLFP and sequencing (Cter-NIb, CP and 3'NCR region) reveals that challenging PPV-Greek belongs to a typical M subgroup. High sequence (both nucleotide 'nt' and amino acid 'aa') homology were found with M type isolates SK68 (nt 99.0% and aa 100%) or PS (nt98.1% and aa 99.3%) and with recombinant isolate BOR 3 (nt 96.0% and aa 97.4%). The PPV-Greek detected in transgenic plum, resistance clone C-5 or susceptible clone C-6 has shown a few mutations, at the position nt 8441 of Cter-NIb and nt 9179 of CP genein C-6 and at the position nt 9068 in C-5. The mutation in CP gene seems to drive from transgenic transcript, suggest the evidence of template switching between CP cistron of challenging virus and homologous transgenic transcript. These

Druh

D - Stať ve sborníku

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Sborník příspěvků z "XVII. české a slovenské konference o ochraně rostlin"

ISBN

80-213-1564-4

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

70-75

Název nakladatele

ČZU

Místo vydání

Praha

Místo konání akce

Praha

Datum konání akce

12. 9. 2006

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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