Spolehlivost molekulárních a biochemických metod pro identifikaci Pseudomonas syringae a dalších pseudomonád z obilnin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F07%3A1346" target="_blank" >RIV/00027006:_____/07:1346 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Reliability of molecular and biochemical methods for identification of Pseudomonas syringae and other Pseudomonads recovered from cereals

Popis výsledku v původním jazyce

Bacteria Pseudomonas spp. harmful on cereals and grasses were investigated, focusing on Pseudomonas syringae. Fluorescent pseudomonads such as Pseudomonas syringae were found most frequently, but P. fluorescens, P. viridiflava and P. putida were also isolated. Approximately 70% of isolates recovered from plants were identified using microbial identification system BIOLOG BACTERIA to species level, but pathovars of P. syringae were not reliably identified. P. syringae pv. syringae strains were confirmedby serological and HR tests on tobacco. Rep-PCR method was applied to all 24 tested isolates and different genetic profiles were obtained. BOX, ERIC and REP primers were used for amplification of genomic DNA. All three sets of primers gave the same results and allowed discrimination of fingerprints into 7 different genotype groups. Members of these groups were subsequently compared to known profiles of selected type culture strains of several pseudomonads. Two of these groups were positi

Název v anglickém jazyce

Reliability of molecular and biochemical methods for identification of Pseudomonas syringae and other Pseudomonads recovered from cereals

Popis výsledku anglicky

Bacteria Pseudomonas spp. harmful on cereals and grasses were investigated, focusing on Pseudomonas syringae. Fluorescent pseudomonads such as Pseudomonas syringae were found most frequently, but P. fluorescens, P. viridiflava and P. putida were also isolated. Approximately 70% of isolates recovered from plants were identified using microbial identification system BIOLOG BACTERIA to species level, but pathovars of P. syringae were not reliably identified. P. syringae pv. syringae strains were confirmedby serological and HR tests on tobacco. Rep-PCR method was applied to all 24 tested isolates and different genetic profiles were obtained. BOX, ERIC and REP primers were used for amplification of genomic DNA. All three sets of primers gave the same results and allowed discrimination of fingerprints into 7 different genotype groups. Members of these groups were subsequently compared to known profiles of selected type culture strains of several pseudomonads. Two of these groups were positi

Druh

D - Stať ve sborníku

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Sborník příspěvků "XVII. česká a slovenská konference o ochraně rostlin"

ISBN

80-213-1564-4

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

138-142

Název nakladatele

ČZU v Praze

Místo vydání

Praha

Místo konání akce

ČZU, Praha 6 - Suchdol

Datum konání akce

12. 9. 2006

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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