Izolace protoplastů a fúze mezi Brassica carinata Braun. a Brassica rapa L.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F07%3AR0076967" target="_blank" >RIV/00027006:_____/07:R0076967 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027006:_____/07:2285

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Protoplast isolation and fusion between Brassica carinata Braun. and Brassica rapa L.

Popis výsledku v původním jazyce

Brassica carinata mesophyll and B. rapa hypocotyl protoplasts were used for fusing experiments using polyethylene glycol (PEG). Treatment with 30% PEG 6000 MW for 10 minutes was more effective, while combinations with 30% PEG 6000 MW/15 min, 30% PEG 4000MW/15 min and 30% PEG 4000 MW for 10 minutes reduced viability of protoplasts and cell wall regeneration, cell division and production of microcolonies. Fusion frequency was about 25% with the treatment of 30% PEG 6000 MW/10 min. Cell colonies forming callus was observed after 14 days of cultivation on liquid B medium with addition of liquid C medium. Microcalli were subsequently transferred to solid E and F medium. Shoot regeneration from calli was induced via indirect organogenesis and somatic embryogenesis on MS hormon free medium. In total 58 calli were obtained from fusions between B. rapa 31/96 and B. carinata line 1; 14 calli produced shoots. According to the morfological diferencies half of shoots were heterocaryonicals. This o

Název v anglickém jazyce

Protoplast isolation and fusion between Brassica carinata Braun. and Brassica rapa L.

Popis výsledku anglicky

Brassica carinata mesophyll and B. rapa hypocotyl protoplasts were used for fusing experiments using polyethylene glycol (PEG). Treatment with 30% PEG 6000 MW for 10 minutes was more effective, while combinations with 30% PEG 6000 MW/15 min, 30% PEG 4000MW/15 min and 30% PEG 4000 MW for 10 minutes reduced viability of protoplasts and cell wall regeneration, cell division and production of microcolonies. Fusion frequency was about 25% with the treatment of 30% PEG 6000 MW/10 min. Cell colonies forming callus was observed after 14 days of cultivation on liquid B medium with addition of liquid C medium. Microcalli were subsequently transferred to solid E and F medium. Shoot regeneration from calli was induced via indirect organogenesis and somatic embryogenesis on MS hormon free medium. In total 58 calli were obtained from fusions between B. rapa 31/96 and B. carinata line 1; 14 calli produced shoots. According to the morfological diferencies half of shoots were heterocaryonicals. This o

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

—

Projekt

<a href="/cs/project/QF4108" target="_blank" >QF4108: Využití techniky fúze protoplastů ve šlechtění hospodářsky významných plodin rodu Brassica, Cucumis a Solanum</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Agricultura Tropica et Subtropica

ISSN

0231-5742

e-ISSN

—

Svazek periodika

—

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

1

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—