Protoplast culture and fusion between Brassica carinata and Brassica napus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F09%3A00000768" target="_blank" >RIV/00027006:_____/09:00000768 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Protoplast culture and fusion between Brassica carinata and Brassica napus

Popis výsledku v původním jazyce

Attempts were made to select in vitro responsible genotypes and to fuse the isolated protoplasts of Brassica carinata and Brassica napus breeding lines, obtained from our previous experiments. Combination of three different PEG concentrations and two different treatment durations (15 and 20 min.) were tested. Our experiments identified several genotypes with satisfactory regeneration ability of calli from protoplast cultures. Proper combinations of concentration and treatment time of PEG determined protoplast fusion frequency between genotypes used. Although the 30% PEG solution was evaluated to be the best concentration, large amount of multifusants, unwanted in practical applications, was detected especially in Petri dishes with longer PEG treatment.In general, 25% PEG combined with 20 minutes treatment duration produced satisfactory fusion frequency and good rate of viability was obtained as well.

Název v anglickém jazyce

Protoplast culture and fusion between Brassica carinata and Brassica napus

Popis výsledku anglicky

Attempts were made to select in vitro responsible genotypes and to fuse the isolated protoplasts of Brassica carinata and Brassica napus breeding lines, obtained from our previous experiments. Combination of three different PEG concentrations and two different treatment durations (15 and 20 min.) were tested. Our experiments identified several genotypes with satisfactory regeneration ability of calli from protoplast cultures. Proper combinations of concentration and treatment time of PEG determined protoplast fusion frequency between genotypes used. Although the 30% PEG solution was evaluated to be the best concentration, large amount of multifusants, unwanted in practical applications, was detected especially in Petri dishes with longer PEG treatment.In general, 25% PEG combined with 20 minutes treatment duration produced satisfactory fusion frequency and good rate of viability was obtained as well.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Agricultura Tropica et Subtropica

ISSN

0231-5742

e-ISSN

—

Svazek periodika

42

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

12

Strana od-do

—

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—