Aplikace proteinových čipů

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F08%3A00000317" target="_blank" >RIV/00027006:_____/08:00000317 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Protein chips applications

Popis výsledku v původním jazyce

The aim of this work was the testing of a diagnostic tool for the detection of allergenic proteins from stored product mites/cockroaches and house dust mites. Species-specific antibodies were tested for specificity and cross-reactivity, and detection limit by ELISA method. In this work, protein chips were manufactured either manually, using a MicroCaster, or robotically, using a SpotArray. The proteins tested were six recombinant Kunitz-type protease inhibitors (PI), two of group A (with anti-cathepsinD and anti-trypsin activity), two of group B (with anti-trypsin and anti-chymotrypsin activity) and two of group C (with anti-cathepsin B activity), and the soybean Bowman-Birk inhibitor. Results of protease binding to each KPI and recognition of allergenic proteases showed the practicability of protein microarrays as a diagnostic tool for detection of mite/insect contaminants in foods and work environment.

Název v anglickém jazyce

Protein chips applications

Popis výsledku anglicky

The aim of this work was the testing of a diagnostic tool for the detection of allergenic proteins from stored product mites/cockroaches and house dust mites. Species-specific antibodies were tested for specificity and cross-reactivity, and detection limit by ELISA method. In this work, protein chips were manufactured either manually, using a MicroCaster, or robotically, using a SpotArray. The proteins tested were six recombinant Kunitz-type protease inhibitors (PI), two of group A (with anti-cathepsinD and anti-trypsin activity), two of group B (with anti-trypsin and anti-chymotrypsin activity) and two of group C (with anti-cathepsin B activity), and the soybean Bowman-Birk inhibitor. Results of protease binding to each KPI and recognition of allergenic proteases showed the practicability of protein microarrays as a diagnostic tool for detection of mite/insect contaminants in foods and work environment.

Druh

C - Kapitola v odborné knize

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

<a href="/cs/project/1P04OC853.003" target="_blank" >1P04OC853.003: Screening trávicích enzymů roztočů jako nových kandidátů do proteinových microarrays</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Action COST 853. Agricultural biomarkers for array technology

ISBN

978-3-033-01770-2

Počet stran výsledku

9

Strana od-do

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Počet stran knihy

104

Název nakladatele

Official Publications Office of European Community (OPOCE)

Místo vydání

Bruxelles

Kód UT WoS kapitoly

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