Feces Derived Allergens of Tyrophagus putrescentiae Reared on Dried Dog Food and Evidence of the Strong Nutritional Interaction between the Mite and Bacillus cereus Producing Protease Bacillolysins and Exo-chitinases

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F16%3A00003576" target="_blank" >RIV/00027006:_____/16:00003576 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/16:70156 RIV/00216208:11310/16:10369113

Výsledek na webu

<a href="http://dx.doi.org/10.3389/fphys.2016.00053" target="_blank" >http://dx.doi.org/10.3389/fphys.2016.00053</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fphys.2016.00053" target="_blank" >10.3389/fphys.2016.00053</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Feces Derived Allergens of Tyrophagus putrescentiae Reared on Dried Dog Food and Evidence of the Strong Nutritional Interaction between the Mite and Bacillus cereus Producing Protease Bacillolysins and Exo-chitinases

Popis výsledku v původním jazyce

The result identifies proteins in excrements of Tyrophagus putrescentiae and reveals interaction between the mite and Bacillus cereus. The 2D-E-MS/MS proteomic analysis identified mite trypsin allergen Tyr p 3, fatty acid-binding protein Tyr p 13 and putative mite allergens ferritin (Grp 30) and (poly)ubiquitins. Tyr p 3 was detected at different positions of the 2D-E; it indicates presence of zymogen at basic pI, and mature-enzyme form and enzyme fragment at acidic pI. The highest protease activity was assigned to trypsin-like proteases; lower activity was assigned to chymotrypsin-like proteases, and the cysteine protease cathepsin B-like had very low activity. Next, in the feces were identified exochitinases and proteases (bacillolysins) of B. cereus. The bacterial proteases are important toxins and exo-chitinases likely contribute to degradation of mite exuviae, mite bodies or food boluses consisting of chitin, including the peritrophic membrane. B. cereus was isolated and identified based on amplification and sequencing of 16S rRNA and motB genes.

Název v anglickém jazyce

Feces Derived Allergens of Tyrophagus putrescentiae Reared on Dried Dog Food and Evidence of the Strong Nutritional Interaction between the Mite and Bacillus cereus Producing Protease Bacillolysins and Exo-chitinases

Popis výsledku anglicky

The result identifies proteins in excrements of Tyrophagus putrescentiae and reveals interaction between the mite and Bacillus cereus. The 2D-E-MS/MS proteomic analysis identified mite trypsin allergen Tyr p 3, fatty acid-binding protein Tyr p 13 and putative mite allergens ferritin (Grp 30) and (poly)ubiquitins. Tyr p 3 was detected at different positions of the 2D-E; it indicates presence of zymogen at basic pI, and mature-enzyme form and enzyme fragment at acidic pI. The highest protease activity was assigned to trypsin-like proteases; lower activity was assigned to chymotrypsin-like proteases, and the cysteine protease cathepsin B-like had very low activity. Next, in the feces were identified exochitinases and proteases (bacillolysins) of B. cereus. The bacterial proteases are important toxins and exo-chitinases likely contribute to degradation of mite exuviae, mite bodies or food boluses consisting of chitin, including the peritrophic membrane. B. cereus was isolated and identified based on amplification and sequencing of 16S rRNA and motB genes.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Physiology

ISSN

1664-042X

e-ISSN

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Svazek periodika

7

Číslo periodika v rámci svazku

February

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

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Kód UT WoS článku

000370693200001

EID výsledku v databázi Scopus

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