Total phenolic content, RAPDs, AFLPs and morphological traits for the analysis of variability in Smallanthus sonchifolius

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F11%3A00001431" target="_blank" >RIV/00027006:_____/11:00001431 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41610/11:51296

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s10722-010-9597-x" target="_blank" >http://dx.doi.org/10.1007/s10722-010-9597-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10722-010-9597-x" target="_blank" >10.1007/s10722-010-9597-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Total phenolic content, RAPDs, AFLPs and morphological traits for the analysis of variability in Smallanthus sonchifolius

Popis výsledku v původním jazyce

In this paper application of RAPDs and AFLPs for the analysis of genetic diversity in a group of 5 Smallanthus sonchifolius landraces is presented. Both methods proceed through the direct analysis of DNA, and their results were compared with the total phenolic content of each landrace and its morphological traits. Using 61 RAPD primers, 85 informative bands were identified, corresponding to 28.7% of polymorphism. In comparsion, only six selected AFLP primer pairs produced 84 informative bands, with a similar percentage of polymorphism (23.4%). Total phenolic content varied twofold among the five landraces analysed, ranging from 3.494 to 6.849 mg/g. Each type of molecular marker resolved two main groups that included the same genotypes, but with different within-group relationships among genotypes. The two groups are consistent with some phenothypic characters but they do not reflect faithfully their geographical origin.

Název v anglickém jazyce

Total phenolic content, RAPDs, AFLPs and morphological traits for the analysis of variability in Smallanthus sonchifolius

Popis výsledku anglicky

In this paper application of RAPDs and AFLPs for the analysis of genetic diversity in a group of 5 Smallanthus sonchifolius landraces is presented. Both methods proceed through the direct analysis of DNA, and their results were compared with the total phenolic content of each landrace and its morphological traits. Using 61 RAPD primers, 85 informative bands were identified, corresponding to 28.7% of polymorphism. In comparsion, only six selected AFLP primer pairs produced 84 informative bands, with a similar percentage of polymorphism (23.4%). Total phenolic content varied twofold among the five landraces analysed, ranging from 3.494 to 6.849 mg/g. Each type of molecular marker resolved two main groups that included the same genotypes, but with different within-group relationships among genotypes. The two groups are consistent with some phenothypic characters but they do not reflect faithfully their geographical origin.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

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Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Genetic Resources and Crop Evolution

ISSN

0925-9864

e-ISSN

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Svazek periodika

58

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

545-551

Kód UT WoS článku

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EID výsledku v databázi Scopus

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