Genetic Diversity Assessment in Amaranth Germplasm using AFLP and ISSR Markers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F14%3A00231398" target="_blank" >RIV/62156489:43510/14:00231398 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.tandfonline.com/doi/abs/10.1080/15427528.2014.921262" target="_blank" >http://www.tandfonline.com/doi/abs/10.1080/15427528.2014.921262</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/15427528.2014.921262" target="_blank" >10.1080/15427528.2014.921262</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Genetic Diversity Assessment in Amaranth Germplasm using AFLP and ISSR Markers

Popis výsledku v původním jazyce

The genetic potential of and relationships among amaranth (Amaranthus spp.) genotypes conserved in gene banks are generally unknown. In this study, nine amplified fragment length polymorphism (AFLP) and 14 inter simple sequence repeats (ISSR) primers were used to study genetic diversity among amaranth species. A total of 92 ISSR loci, of which 86 were polymorphic, were detected. The bands ranged from 300 bp to 2000 bp with polymorphic information content (PIC) of 047. The AFLP primer combinations revealed a total of 648 bands, with 489 as the number of polymorphic loci. The fragments ranged from 52 bp to 423 bp in length with PIC of 0.48. The correlation between genetic similarity based on ISSR and AFLP was 0.77: thus, 59% of the relationship was explained. The cluster analysis revealed distinct groups of amaranth genotypes. Therefore, the use of molecular markers would be valuable for effective utilization of amaranth in breeding programs.

Název v anglickém jazyce

Genetic Diversity Assessment in Amaranth Germplasm using AFLP and ISSR Markers

Popis výsledku anglicky

The genetic potential of and relationships among amaranth (Amaranthus spp.) genotypes conserved in gene banks are generally unknown. In this study, nine amplified fragment length polymorphism (AFLP) and 14 inter simple sequence repeats (ISSR) primers were used to study genetic diversity among amaranth species. A total of 92 ISSR loci, of which 86 were polymorphic, were detected. The bands ranged from 300 bp to 2000 bp with polymorphic information content (PIC) of 047. The AFLP primer combinations revealed a total of 648 bands, with 489 as the number of polymorphic loci. The fragments ranged from 52 bp to 423 bp in length with PIC of 0.48. The correlation between genetic similarity based on ISSR and AFLP was 0.77: thus, 59% of the relationship was explained. The cluster analysis revealed distinct groups of amaranth genotypes. Therefore, the use of molecular markers would be valuable for effective utilization of amaranth in breeding programs.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

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Projekt

—

Návaznosti

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Crop Improvement

ISSN

1542-7528

e-ISSN

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Svazek periodika

28

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

518-529

Kód UT WoS článku

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EID výsledku v databázi Scopus

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