Using of AFLP to evaluate gamma-irradiated amaranth mutants

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F13%3A00002833" target="_blank" >RIV/00027006:_____/13:00002833 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.2298/GENSR1303825L" target="_blank" >http://dx.doi.org/10.2298/GENSR1303825L</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2298/GENSR1303825L" target="_blank" >10.2298/GENSR1303825L</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Using of AFLP to evaluate gamma-irradiated amaranth mutants

Popis výsledku v původním jazyce

To determine which of several gamma-irradiated mutants of amaranth Ficha cultivar and K-433 hybrid are most genetically similar to their non-irradiated control genotypes, we performed amplified fragment length polymorphism (AFLP) based analysis. A totalof 40 selective primer combinations were used in reported analyses. First analyses of gamma-irradiated amaranth mutant lines were done used the AFLP. In the study, primers with the differentiation ability for all analysed mutant lines are reported. The very specific changes in the mutant lines´ non-coding regions based on AFLP length polymorphism were analysed. Mutant lines of the Ficha cultivar (C15, C26, C27, C82, C236) shared a genetic dissimilarity of 0,11 and their ISSR profiles are more similar tothe Ficha than those of K-433 hybrid mutant lines. The K-433 mutant lines (D54, D279, D282) shared genetic dissimilarity of 0,534 but are more distinct to their control plant as a whole, as those of the Ficha mutant lines. Different AFLP

Název v anglickém jazyce

Using of AFLP to evaluate gamma-irradiated amaranth mutants

Popis výsledku anglicky

To determine which of several gamma-irradiated mutants of amaranth Ficha cultivar and K-433 hybrid are most genetically similar to their non-irradiated control genotypes, we performed amplified fragment length polymorphism (AFLP) based analysis. A totalof 40 selective primer combinations were used in reported analyses. First analyses of gamma-irradiated amaranth mutant lines were done used the AFLP. In the study, primers with the differentiation ability for all analysed mutant lines are reported. The very specific changes in the mutant lines´ non-coding regions based on AFLP length polymorphism were analysed. Mutant lines of the Ficha cultivar (C15, C26, C27, C82, C236) shared a genetic dissimilarity of 0,11 and their ISSR profiles are more similar tothe Ficha than those of K-433 hybrid mutant lines. The K-433 mutant lines (D54, D279, D282) shared genetic dissimilarity of 0,534 but are more distinct to their control plant as a whole, as those of the Ficha mutant lines. Different AFLP

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Genetika-Belgrade

ISSN

0534-0012

e-ISSN

—

Svazek periodika

45

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CS - Srbsko a Černá Hora

Počet stran výsledku

11

Strana od-do

825-835

Kód UT WoS článku

000330186600019

EID výsledku v databázi Scopus

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