Rapid diagnosis of two common stored-product predatory mite species based on species-specific PCR

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F16%3A00003696" target="_blank" >RIV/00027006:_____/16:00003696 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jspr.2016.08.006" target="_blank" >http://dx.doi.org/10.1016/j.jspr.2016.08.006</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jspr.2016.08.006" target="_blank" >10.1016/j.jspr.2016.08.006</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Rapid diagnosis of two common stored-product predatory mite species based on species-specific PCR

Popis výsledku v původním jazyce

Cheyletus malaccensis Oudemans and Cheyletus eruditus (Schrank) are two sibling species of the Cheyletus genus. Both species are beneficial predators of stored product pests. It is difficult to distinguish them based only on morphological characteristics. In this study, we describe species-specific PCR for molecular identification of C. malaccensis and C. eruditus. Species-specific primer pairs were designed on the basis of variations in the mtDNA COI barcode sequences. A 403 bp-specific fragment of C. malaccensis and a 421 bp absolute product of C. eruditus were generated by their respective primer pairs, while no such diagnostic bands were present in any of the nine pest mite species tested. The results of the sensitivity test demonstrated that the detection limit of DNA template concentration was 1 ng/µL for both C. malaccensis and C. eruditus. This study exhibited high specificity, reliability, rapid and low cost for mite specie diagnosis. Our data show that species-specific PCR is a powerful tool that can be used for identification of C. malaccensis and C. eruditus.

Název v anglickém jazyce

Rapid diagnosis of two common stored-product predatory mite species based on species-specific PCR

Popis výsledku anglicky

Cheyletus malaccensis Oudemans and Cheyletus eruditus (Schrank) are two sibling species of the Cheyletus genus. Both species are beneficial predators of stored product pests. It is difficult to distinguish them based only on morphological characteristics. In this study, we describe species-specific PCR for molecular identification of C. malaccensis and C. eruditus. Species-specific primer pairs were designed on the basis of variations in the mtDNA COI barcode sequences. A 403 bp-specific fragment of C. malaccensis and a 421 bp absolute product of C. eruditus were generated by their respective primer pairs, while no such diagnostic bands were present in any of the nine pest mite species tested. The results of the sensitivity test demonstrated that the detection limit of DNA template concentration was 1 ng/µL for both C. malaccensis and C. eruditus. This study exhibited high specificity, reliability, rapid and low cost for mite specie diagnosis. Our data show that species-specific PCR is a powerful tool that can be used for identification of C. malaccensis and C. eruditus.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Stored Products Research

ISSN

0022-474X

e-ISSN

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Svazek periodika

69

Číslo periodika v rámci svazku

October

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

4

Strana od-do

213-216

Kód UT WoS článku

000390514900032

EID výsledku v databázi Scopus

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