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Detection of Clavibacter michiganensis subsp. Sepedonicus, the causal agent of potato ring rot, in the breeding and propagation materials of the three-stage control process

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F16%3A00003755" target="_blank" >RIV/00027006:_____/16:00003755 - isvavai.cz</a>

  • Výsledek na webu

  • DOI - Digital Object Identifier

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Detection of Clavibacter michiganensis subsp. Sepedonicus, the causal agent of potato ring rot, in the breeding and propagation materials of the three-stage control process

  • Popis výsledku v původním jazyce

    To maintain Clavibacter michiganensis subsp. sepedonicus-free (Cms-free) potato genetic resources, a three-stage process of checking limited lots of initial and new breeding potato materials was designed for small breeders in terms of costs, personnel and facilities. For an intensified evaluation of the presence of Cms in different vegetative stages within one growing season a mixture of 10 randomly selected artificially infected and healthy tubers of individual cultivars and breeding materials were prepared. In the first stage, a visual assessment of the vascular vessels of mother tubers was performed and at least 20% of each material was deemed positive, indicating that it should be discarded. In the second stage, one half of mother tubers were grown in the greenhouse and the other in a netting house. Plants in bloom and daughter tubers were evaluated. Within the second stage 1.4%–28% of each individual potato material could be excluded based on double-antibody sandwich enzyme-linked immunosorbent assays and SYBR Green real-time PCR assays, including a melting point analysis using PSA 1/R and CelA F/R primer sets and biological tests on aubergines. In the third stage, in vitro plants transferred from potato resources that tested negative in the previous stages were evaluated using the methods used in the second stage. The specificity of the antibodies and the two primer sets, PSA1/R and CelA F/R, were evaluated with 100% success.

  • Název v anglickém jazyce

    Detection of Clavibacter michiganensis subsp. Sepedonicus, the causal agent of potato ring rot, in the breeding and propagation materials of the three-stage control process

  • Popis výsledku anglicky

    To maintain Clavibacter michiganensis subsp. sepedonicus-free (Cms-free) potato genetic resources, a three-stage process of checking limited lots of initial and new breeding potato materials was designed for small breeders in terms of costs, personnel and facilities. For an intensified evaluation of the presence of Cms in different vegetative stages within one growing season a mixture of 10 randomly selected artificially infected and healthy tubers of individual cultivars and breeding materials were prepared. In the first stage, a visual assessment of the vascular vessels of mother tubers was performed and at least 20% of each material was deemed positive, indicating that it should be discarded. In the second stage, one half of mother tubers were grown in the greenhouse and the other in a netting house. Plants in bloom and daughter tubers were evaluated. Within the second stage 1.4%–28% of each individual potato material could be excluded based on double-antibody sandwich enzyme-linked immunosorbent assays and SYBR Green real-time PCR assays, including a melting point analysis using PSA 1/R and CelA F/R primer sets and biological tests on aubergines. In the third stage, in vitro plants transferred from potato resources that tested negative in the previous stages were evaluated using the methods used in the second stage. The specificity of the antibodies and the two primer sets, PSA1/R and CelA F/R, were evaluated with 100% success.

Klasifikace

  • Druh

    J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

  • CEP obor

    GF - Choroby, škůdci, plevely a ochrana rostlin

  • OECD FORD obor

Návaznosti výsledku

  • Projekt

    <a href="/cs/project/QJ1310218" target="_blank" >QJ1310218: Snížení rizika výskytu původce bakteriální kroužkovitosti bramboru v šlechtitelském a množitelském materiálu.</a><br>

  • Návaznosti

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Ostatní

  • Rok uplatnění

    2016

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název periodika

    Journal of Phytopathology and Pest Management

  • ISSN

    2356-6507

  • e-ISSN

  • Svazek periodika

    3

  • Číslo periodika v rámci svazku

    2

  • Stát vydavatele periodika

    EG - Egyptská arabská republika

  • Počet stran výsledku

    16

  • Strana od-do

    48-63

  • Kód UT WoS článku

  • EID výsledku v databázi Scopus