Minor cereals exhibit superior antioxidant effects on human epithelial cells compared to common wheat cultivars

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F19%3A00005364" target="_blank" >RIV/00027006:_____/19:00005364 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/abs/pii/S0733521018304430" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S0733521018304430</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jcs.2018.12.006" target="_blank" >10.1016/j.jcs.2018.12.006</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Minor cereals exhibit superior antioxidant effects on human epithelial cells compared to common wheat cultivars

Popis výsledku v původním jazyce

In the present study, various minor cereals including rye, oat, spelt, primitive and wild wheat species (20 genotypes each) were tested for their antioxidative effects in cell biological studies, and the effects were compared with the effects of 12 common wheat genotypes. Ethanol soluble extracts of grains were used and studied in human cancer-derived cell lines including HeLa (cervical) and HCT116 (colorectal) cell lines by analyzing cellular levels of reactive oxygen species (ROS), mitochondrial health, DNA damage and cell survival. The cereal genotypes were also examined for the total phenolic concentration (TPC) and for the relationship between oxidative cell damage and TPC values. Most genotypes of the minor cereal species showed superior antioxidant effects in human cell cultures compared to the common wheat genotypes. Many of the minor cereal genotypes with high antioxidant capacities protected DNA and mitochondrial damage and prevented cell death in HeLa cells and colon-derived HCT116 human epithelial cells. There was no significant relationship between TPC values and oxidative cell damage. However, common wheat genotypes had, on average, lower TPC values. The results show that minor cereals contain potentially high levels of certain antioxidant substances which might be useful in preventing oxidative damage in cellular systems.

Název v anglickém jazyce

Minor cereals exhibit superior antioxidant effects on human epithelial cells compared to common wheat cultivars

Popis výsledku anglicky

In the present study, various minor cereals including rye, oat, spelt, primitive and wild wheat species (20 genotypes each) were tested for their antioxidative effects in cell biological studies, and the effects were compared with the effects of 12 common wheat genotypes. Ethanol soluble extracts of grains were used and studied in human cancer-derived cell lines including HeLa (cervical) and HCT116 (colorectal) cell lines by analyzing cellular levels of reactive oxygen species (ROS), mitochondrial health, DNA damage and cell survival. The cereal genotypes were also examined for the total phenolic concentration (TPC) and for the relationship between oxidative cell damage and TPC values. Most genotypes of the minor cereal species showed superior antioxidant effects in human cell cultures compared to the common wheat genotypes. Many of the minor cereal genotypes with high antioxidant capacities protected DNA and mitochondrial damage and prevented cell death in HeLa cells and colon-derived HCT116 human epithelial cells. There was no significant relationship between TPC values and oxidative cell damage. However, common wheat genotypes had, on average, lower TPC values. The results show that minor cereals contain potentially high levels of certain antioxidant substances which might be useful in preventing oxidative damage in cellular systems.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

—

Návaznosti

R - Projekt Ramcoveho programu EK

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Cereal Science

ISSN

0733-5210

e-ISSN

1095-9963

Svazek periodika

85

Číslo periodika v rámci svazku

JAN 2019

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

143-152

Kód UT WoS článku

000457950900022

EID výsledku v databázi Scopus

2-s2.0-85059325868