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Carlina oxide from Carlina acaulis root essential oil acts as a potent mosquito larvicide

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F19%3A00005388" target="_blank" >RIV/00027006:_____/19:00005388 - isvavai.cz</a>

  • Nalezeny alternativní kódy

    RIV/60460709:41210/19:80923

  • Výsledek na webu

    <a href="https://www.sciencedirect.com/science/article/abs/pii/S0926669019303711" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S0926669019303711</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.indcrop.2019.05.037" target="_blank" >10.1016/j.indcrop.2019.05.037</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Carlina oxide from Carlina acaulis root essential oil acts as a potent mosquito larvicide

  • Popis výsledku v původním jazyce

    Developing effective larvicides for mosquito control is being challenging due to the quick development of resistance in targeted vectors. Botanical products can help, due to their multiple mechanisms of action coupled with eco-friendly features. Carlina acaulis (Asteraceae) is an Alpine perennial herb used as a traditional remedy and food. Its root essential oil (EO) has antimicrobial and antitrypanosomal properties, and is currently listed among botanicals employable in food supplements. Its insecticidal activity has not been explored so far. Here, after analysing the C. acaulis EO chemical composition by GC-MS and NMR, we detected extremely high larvicidal activity of this EO and its main constituent, carlina oxide, against Culex quinquefasciatus larvae by using the standard WHO protocol. LC50 were 1.31 and 1.39 mu g mL(-1), respectively. 24-h exposure to both products triggered significant mortality rates for five days post-treatment. Larvicidal tests on a wider scale confirmed > 95% larvicidal effectiveness of the EO and carlina oxide tested at 1.25 mu g L-1. Their non-target impact was evaluated through experiments on Daphnia magna adults. Both showed significantly lower toxicity if compared to cypermethrin. To shed light on the modes of action, carlina oxide was tested for anti-acetylcholinesterase activity by the Ellman method, with lower performances over galantamine. A moderate antioxidant potential was observed using DPPH and ABTS assays, since it has a role for preserving the shelf-life of herbal-based insecticides. Finally, cytotoxicity on vertebrate cells was noted, testing carlina oxide on human dermis, HCT116 and MDA-MB231 cell lines by MTT assay. Overall, the outstanding toxicity of the tested products make them excellent candidates to develop novel mosquito larvicides for real-world applications.

  • Název v anglickém jazyce

    Carlina oxide from Carlina acaulis root essential oil acts as a potent mosquito larvicide

  • Popis výsledku anglicky

    Developing effective larvicides for mosquito control is being challenging due to the quick development of resistance in targeted vectors. Botanical products can help, due to their multiple mechanisms of action coupled with eco-friendly features. Carlina acaulis (Asteraceae) is an Alpine perennial herb used as a traditional remedy and food. Its root essential oil (EO) has antimicrobial and antitrypanosomal properties, and is currently listed among botanicals employable in food supplements. Its insecticidal activity has not been explored so far. Here, after analysing the C. acaulis EO chemical composition by GC-MS and NMR, we detected extremely high larvicidal activity of this EO and its main constituent, carlina oxide, against Culex quinquefasciatus larvae by using the standard WHO protocol. LC50 were 1.31 and 1.39 mu g mL(-1), respectively. 24-h exposure to both products triggered significant mortality rates for five days post-treatment. Larvicidal tests on a wider scale confirmed > 95% larvicidal effectiveness of the EO and carlina oxide tested at 1.25 mu g L-1. Their non-target impact was evaluated through experiments on Daphnia magna adults. Both showed significantly lower toxicity if compared to cypermethrin. To shed light on the modes of action, carlina oxide was tested for anti-acetylcholinesterase activity by the Ellman method, with lower performances over galantamine. A moderate antioxidant potential was observed using DPPH and ABTS assays, since it has a role for preserving the shelf-life of herbal-based insecticides. Finally, cytotoxicity on vertebrate cells was noted, testing carlina oxide on human dermis, HCT116 and MDA-MB231 cell lines by MTT assay. Overall, the outstanding toxicity of the tested products make them excellent candidates to develop novel mosquito larvicides for real-world applications.

Klasifikace

  • Druh

    J<sub>imp</sub> - Článek v periodiku v databázi Web of Science

  • CEP obor

  • OECD FORD obor

    40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Návaznosti výsledku

  • Projekt

  • Návaznosti

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Ostatní

  • Rok uplatnění

    2019

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název periodika

    Industrial Crops and Products

  • ISSN

    0926-6690

  • e-ISSN

    1872-633X

  • Svazek periodika

    137

  • Číslo periodika v rámci svazku

    OCT 1 2019

  • Stát vydavatele periodika

    NL - Nizozemsko

  • Počet stran výsledku

    11

  • Strana od-do

    356-366

  • Kód UT WoS článku

    000473376800044

  • EID výsledku v databázi Scopus

    2-s2.0-85065894236