Carlina oxide from Carlina acaulis root essential oil acts as a potent mosquito larvicide

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F19%3A00005388" target="_blank" >RIV/00027006:_____/19:00005388 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/19:80923

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/abs/pii/S0926669019303711" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S0926669019303711</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.indcrop.2019.05.037" target="_blank" >10.1016/j.indcrop.2019.05.037</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Carlina oxide from Carlina acaulis root essential oil acts as a potent mosquito larvicide

Popis výsledku v původním jazyce

Developing effective larvicides for mosquito control is being challenging due to the quick development of resistance in targeted vectors. Botanical products can help, due to their multiple mechanisms of action coupled with eco-friendly features. Carlina acaulis (Asteraceae) is an Alpine perennial herb used as a traditional remedy and food. Its root essential oil (EO) has antimicrobial and antitrypanosomal properties, and is currently listed among botanicals employable in food supplements. Its insecticidal activity has not been explored so far. Here, after analysing the C. acaulis EO chemical composition by GC-MS and NMR, we detected extremely high larvicidal activity of this EO and its main constituent, carlina oxide, against Culex quinquefasciatus larvae by using the standard WHO protocol. LC50 were 1.31 and 1.39 mu g mL(-1), respectively. 24-h exposure to both products triggered significant mortality rates for five days post-treatment. Larvicidal tests on a wider scale confirmed > 95% larvicidal effectiveness of the EO and carlina oxide tested at 1.25 mu g L-1. Their non-target impact was evaluated through experiments on Daphnia magna adults. Both showed significantly lower toxicity if compared to cypermethrin. To shed light on the modes of action, carlina oxide was tested for anti-acetylcholinesterase activity by the Ellman method, with lower performances over galantamine. A moderate antioxidant potential was observed using DPPH and ABTS assays, since it has a role for preserving the shelf-life of herbal-based insecticides. Finally, cytotoxicity on vertebrate cells was noted, testing carlina oxide on human dermis, HCT116 and MDA-MB231 cell lines by MTT assay. Overall, the outstanding toxicity of the tested products make them excellent candidates to develop novel mosquito larvicides for real-world applications.

Název v anglickém jazyce

Carlina oxide from Carlina acaulis root essential oil acts as a potent mosquito larvicide

Popis výsledku anglicky

Developing effective larvicides for mosquito control is being challenging due to the quick development of resistance in targeted vectors. Botanical products can help, due to their multiple mechanisms of action coupled with eco-friendly features. Carlina acaulis (Asteraceae) is an Alpine perennial herb used as a traditional remedy and food. Its root essential oil (EO) has antimicrobial and antitrypanosomal properties, and is currently listed among botanicals employable in food supplements. Its insecticidal activity has not been explored so far. Here, after analysing the C. acaulis EO chemical composition by GC-MS and NMR, we detected extremely high larvicidal activity of this EO and its main constituent, carlina oxide, against Culex quinquefasciatus larvae by using the standard WHO protocol. LC50 were 1.31 and 1.39 mu g mL(-1), respectively. 24-h exposure to both products triggered significant mortality rates for five days post-treatment. Larvicidal tests on a wider scale confirmed > 95% larvicidal effectiveness of the EO and carlina oxide tested at 1.25 mu g L-1. Their non-target impact was evaluated through experiments on Daphnia magna adults. Both showed significantly lower toxicity if compared to cypermethrin. To shed light on the modes of action, carlina oxide was tested for anti-acetylcholinesterase activity by the Ellman method, with lower performances over galantamine. A moderate antioxidant potential was observed using DPPH and ABTS assays, since it has a role for preserving the shelf-life of herbal-based insecticides. Finally, cytotoxicity on vertebrate cells was noted, testing carlina oxide on human dermis, HCT116 and MDA-MB231 cell lines by MTT assay. Overall, the outstanding toxicity of the tested products make them excellent candidates to develop novel mosquito larvicides for real-world applications.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Industrial Crops and Products

ISSN

0926-6690

e-ISSN

1872-633X

Svazek periodika

137

Číslo periodika v rámci svazku

OCT 1 2019

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

356-366

Kód UT WoS článku

000473376800044

EID výsledku v databázi Scopus

2-s2.0-85065894236