Methods of Thermal Analysis as a Tool to Develop Cryopreservation Protocols of Vegetatively Propagated Crops

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F20%3A10144247" target="_blank" >RIV/00027006:_____/20:10144247 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.intechopen.com/books/cryopreservation-current-advances-and-evaluations/methods-of-thermal-analysis-as-a-tool-to-develop-cryopreservation-protocols-of-vegetatively-propagat" target="_blank" >https://www.intechopen.com/books/cryopreservation-current-advances-and-evaluations/methods-of-thermal-analysis-as-a-tool-to-develop-cryopreservation-protocols-of-vegetatively-propagat</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5772/intechopen.89723" target="_blank" >10.5772/intechopen.89723</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Methods of Thermal Analysis as a Tool to Develop Cryopreservation Protocols of Vegetatively Propagated Crops

Popis výsledku v původním jazyce

Cryopreservation is considered to be a reliable biotechnological tool for the long-term conservation of vegetatively propagated plant germplasm. The technique is based on freezing plant tissues at an ultra-low temperature. However, high water content in plant tissue can result in injury during the cooling and thawing processes. Water behaviour in the process of cryopreservation can be assessed by the use of thermal analysis method. This chapter demonstrates how the use of heat flux type differential scanning calorimetry (DSC) thermal analysis methods such as Standard DSC, Temperature Modulated DSC (TMDSC) and Quasi-Isothermal Temperature Modulated DSC (QITMDSC) can be used to assess the amount of freezable water and verify if the tissue being used has reached glass transition as well as analysing the thermal events during cooling and freezing to reduce crystallization and damage by frost. Here, you can find a guide on how these thermal analysis methods can be applied, through concrete examples of each method and their use in the development of a more reliable and precise cryopreservation protocol for vegetatively propagated plant species.

Název v anglickém jazyce

Methods of Thermal Analysis as a Tool to Develop Cryopreservation Protocols of Vegetatively Propagated Crops

Popis výsledku anglicky

Cryopreservation is considered to be a reliable biotechnological tool for the long-term conservation of vegetatively propagated plant germplasm. The technique is based on freezing plant tissues at an ultra-low temperature. However, high water content in plant tissue can result in injury during the cooling and thawing processes. Water behaviour in the process of cryopreservation can be assessed by the use of thermal analysis method. This chapter demonstrates how the use of heat flux type differential scanning calorimetry (DSC) thermal analysis methods such as Standard DSC, Temperature Modulated DSC (TMDSC) and Quasi-Isothermal Temperature Modulated DSC (QITMDSC) can be used to assess the amount of freezable water and verify if the tissue being used has reached glass transition as well as analysing the thermal events during cooling and freezing to reduce crystallization and damage by frost. Here, you can find a guide on how these thermal analysis methods can be applied, through concrete examples of each method and their use in the development of a more reliable and precise cryopreservation protocol for vegetatively propagated plant species.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

40401 - Agricultural biotechnology and food biotechnology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Cryopreservation - Current Advances and Evaluations

ISBN

978-1-83880-205-9

Počet stran výsledku

13

Strana od-do

161-173

Počet stran knihy

190

Název nakladatele

IntechOpen

Místo vydání

London

Kód UT WoS kapitoly

—