Thermal analysis methods as a tool for development of cryopreservation protocols

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F20%3A10149469" target="_blank" >RIV/00027006:_____/20:10149469 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027006:_____/19:00005666

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Thermal analysis methods as a tool for development of cryopreservation protocols

Popis výsledku v původním jazyce

Understanding the behaviour of the water content in plant tissues during cooling and heating is a key factor in developing a reliable cryopreservation protocol as high water content during these processes can result in sample injury. Differential Scanning Calorimetry (DSC) thermal analysis methods such as standard DSC (DSC), Temperature Modulated DSC (TMDSC) and Quasi-isothermal Temperature Modulated DSC (QITMDSC) represent a viable tool that can improve the knowledge of the thermal events and so help to reduce ice crystallization and cell damage by frost and so contribute in the development of more efficient and reliable cryopreservation protocols. The standard DSC was used for routine measurement when dealing with samples with known thermal properties and non-overlapping thermal events. Amount of freezable water or glass transition were detected by the standard DSC after specific sample dehydration. MDSC method was used for analysing samples with overlapping thermal events. The method helped to separate a total heat-flow signal into thermodynamic and kinetic events. QITMDSC method was used for the exact measurement of heat capacity in equilibrated conditions, that help to identify the sample state of matter. Here we intend to demonstrate how the use of these DSC thermal analysis methods can be applied in the development of cryopreservation protocols, what DSC methods are appropriate for samples with specific thermal characteristics, what thermal events can endanger sample cryopreservation stability, and what results can be expected using a specific thermal analysis method.

Název v anglickém jazyce

Thermal analysis methods as a tool for development of cryopreservation protocols

Popis výsledku anglicky

Understanding the behaviour of the water content in plant tissues during cooling and heating is a key factor in developing a reliable cryopreservation protocol as high water content during these processes can result in sample injury. Differential Scanning Calorimetry (DSC) thermal analysis methods such as standard DSC (DSC), Temperature Modulated DSC (TMDSC) and Quasi-isothermal Temperature Modulated DSC (QITMDSC) represent a viable tool that can improve the knowledge of the thermal events and so help to reduce ice crystallization and cell damage by frost and so contribute in the development of more efficient and reliable cryopreservation protocols. The standard DSC was used for routine measurement when dealing with samples with known thermal properties and non-overlapping thermal events. Amount of freezable water or glass transition were detected by the standard DSC after specific sample dehydration. MDSC method was used for analysing samples with overlapping thermal events. The method helped to separate a total heat-flow signal into thermodynamic and kinetic events. QITMDSC method was used for the exact measurement of heat capacity in equilibrated conditions, that help to identify the sample state of matter. Here we intend to demonstrate how the use of these DSC thermal analysis methods can be applied in the development of cryopreservation protocols, what DSC methods are appropriate for samples with specific thermal characteristics, what thermal events can endanger sample cryopreservation stability, and what results can be expected using a specific thermal analysis method.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40401 - Agricultural biotechnology and food biotechnology

Projekt

<a href="/cs/project/QK1910277" target="_blank" >QK1910277: Využití metody kryoprezervace pro zefektivnění šlechtitelského procesu hospodářsky významných zemědělských plodin a uchování lesních dřevin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů