Detection and quantification of Melissococcus plutonius in honey bee workers exposed to European foulbrood in Czechia through conventional PCR, qPCR, and barcode sequencing

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F20%3A10146543" target="_blank" >RIV/00027006:_____/20:10146543 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.tandfonline.com/doi/abs/10.1080/00218839.2019.1685148?journalCode=tjar20" target="_blank" >https://www.tandfonline.com/doi/abs/10.1080/00218839.2019.1685148?journalCode=tjar20</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/00218839.2019.1685148" target="_blank" >10.1080/00218839.2019.1685148</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Detection and quantification of Melissococcus plutonius in honey bee workers exposed to European foulbrood in Czechia through conventional PCR, qPCR, and barcode sequencing

Popis výsledku v původním jazyce

Melissococcus plutonius is the causative agent of European foulbrood (EFB), an important bacterial disease of honey bee larvae, and various methods have been developed for the detection of this disease. Adult bees are not affected but spread M. plutonius among beehives and serve as a diagnostic tool to detect EFB. Melissococcus plutonius detection based on conventional polymerase chain reaction (PCR) (16S DNA), qPCR (sodA), and barcode sequencing of the 16S RNA V4 region in worker bees from colonies with and without clinical symptoms were compared. We validated these detection tools in terms of the presence/absence of clinical signs of the disease. The comparison of the PCR- and qPCR-based methods showed their usability for confirmation of the disease in both colonies with and without clinical symptoms. Our results revealed that qPCR was more suitable for the confirmation of clinical EFB than conventional PCR and that conventional PCR was better for general screening, including the screening of asymptomatic colonies, than qPCR. Redundancy analyses (tbRDAs) of the microbiome composition showed that the detection limit-based qPCR of M. plutonius revealed a greater variability in the microbiome profiles of worker bees compared with that explained by clinical signs of the disease and PCR detection as factors. The presence of secondary invaders (Paenibacillus alvei and Enterococcus) was positively correlated with an increase in the profile of M. plutonius in the worker bee microbiome, whereas Apibacter adventoris and Bartonella apis were negatively correlated. Both types of correlations were found among Lactobacilli. (C) 2019, (C) 2019 International Bee Research Association.

Název v anglickém jazyce

Detection and quantification of Melissococcus plutonius in honey bee workers exposed to European foulbrood in Czechia through conventional PCR, qPCR, and barcode sequencing

Popis výsledku anglicky

Melissococcus plutonius is the causative agent of European foulbrood (EFB), an important bacterial disease of honey bee larvae, and various methods have been developed for the detection of this disease. Adult bees are not affected but spread M. plutonius among beehives and serve as a diagnostic tool to detect EFB. Melissococcus plutonius detection based on conventional polymerase chain reaction (PCR) (16S DNA), qPCR (sodA), and barcode sequencing of the 16S RNA V4 region in worker bees from colonies with and without clinical symptoms were compared. We validated these detection tools in terms of the presence/absence of clinical signs of the disease. The comparison of the PCR- and qPCR-based methods showed their usability for confirmation of the disease in both colonies with and without clinical symptoms. Our results revealed that qPCR was more suitable for the confirmation of clinical EFB than conventional PCR and that conventional PCR was better for general screening, including the screening of asymptomatic colonies, than qPCR. Redundancy analyses (tbRDAs) of the microbiome composition showed that the detection limit-based qPCR of M. plutonius revealed a greater variability in the microbiome profiles of worker bees compared with that explained by clinical signs of the disease and PCR detection as factors. The presence of secondary invaders (Paenibacillus alvei and Enterococcus) was positively correlated with an increase in the profile of M. plutonius in the worker bee microbiome, whereas Apibacter adventoris and Bartonella apis were negatively correlated. Both types of correlations were found among Lactobacilli. (C) 2019, (C) 2019 International Bee Research Association.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/TH02030317" target="_blank" >TH02030317: Vývoj nástrojů a pomůcek pro včasnou detekci hniloby včelího plodu</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF APICULTURAL RESEARCH

ISSN

0021-8839

e-ISSN

—

Svazek periodika

59

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

503-514

Kód UT WoS článku

000495156300001

EID výsledku v databázi Scopus

2-s2.0-85074821928