A scientific note on the comparison of PCR based quantification methods of Melissococcus plutonius in honey bees

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F20%3A10149609" target="_blank" >RIV/00027006:_____/20:10149609 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/20:10442458 RIV/60460709:41210/20:88583

Výsledek na webu

<a href="https://www.tandfonline.com/toc/tjar20/60/2" target="_blank" >https://www.tandfonline.com/toc/tjar20/60/2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/00218839.2020.1844466" target="_blank" >10.1080/00218839.2020.1844466</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A scientific note on the comparison of PCR based quantification methods of Melissococcus plutonius in honey bees

Popis výsledku v původním jazyce

Melissococcus plutonius is the causative agent of European foulbrood of honey bee larvae (Apis mellifera). The early diagnosis of disease is necessary for its elimination. Previously, we found false-positive results for PCR detection of M. plutonius in honey bee workers by qPCR. Here we report the comparison of PCR methods for quantification of M. plutonius in the bacteria spiked samples of honey bee workers and detection limits to eliminate false-positive results. We compare available primers of qPCR and PCR detection. The obtained detection limit was 10(2) copies of M. plutonius. The results of this comparison show conventional PCR detection using EFB_primers as a suitable method to confirm visual observation of European foulbrood using samples of honey bee workers. Based on our estimation, the numbers of M. plutonius should be quantified by copies of the Na thorn /H thorn 50 antiporter marker (MP_primers) in honey bee worker samples

Název v anglickém jazyce

A scientific note on the comparison of PCR based quantification methods of Melissococcus plutonius in honey bees

Popis výsledku anglicky

Melissococcus plutonius is the causative agent of European foulbrood of honey bee larvae (Apis mellifera). The early diagnosis of disease is necessary for its elimination. Previously, we found false-positive results for PCR detection of M. plutonius in honey bee workers by qPCR. Here we report the comparison of PCR methods for quantification of M. plutonius in the bacteria spiked samples of honey bee workers and detection limits to eliminate false-positive results. We compare available primers of qPCR and PCR detection. The obtained detection limit was 10(2) copies of M. plutonius. The results of this comparison show conventional PCR detection using EFB_primers as a suitable method to confirm visual observation of European foulbrood using samples of honey bee workers. Based on our estimation, the numbers of M. plutonius should be quantified by copies of the Na thorn /H thorn 50 antiporter marker (MP_primers) in honey bee worker samples

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/TH02030317" target="_blank" >TH02030317: Vývoj nástrojů a pomůcek pro včasnou detekci hniloby včelího plodu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Apicultural Research

ISSN

0021-8839

e-ISSN

—

Svazek periodika

60

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

5

Strana od-do

255-259

Kód UT WoS článku

000592035000001

EID výsledku v databázi Scopus

2-s2.0-85096603370