Plant virome analysis in search for new viruses: experience from CRI Prague, Czech republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F20%3A10149403" target="_blank" >RIV/00027006:_____/20:10149403 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Plant virome analysis in search for new viruses: experience from CRI Prague, Czech republic

Popis výsledku v původním jazyce

Viromes of fruit trees, vegetables, grapevines, and ornamentals were studied using highthroughput sequencing. Total RNA was isolated from plant leaves, ribosomal RNA was removed and libraries for Illumina technology were prepared. Sequencing was done in NovaSeq instrument for 2 x 150 nt. Data were processed using Geneious, pipeline included de novo assembly, contigs search by TBLASTX for plant viruses using a local database. Reads were then mapped back to contigs. A number of viruses were identified in analyzed samples: six viruses and two viroids in grapevines; two viruses in fruit trees; 11 viruses including four novel viruses in ornamentals.

Název v anglickém jazyce

Plant virome analysis in search for new viruses: experience from CRI Prague, Czech republic

Popis výsledku anglicky

Viromes of fruit trees, vegetables, grapevines, and ornamentals were studied using highthroughput sequencing. Total RNA was isolated from plant leaves, ribosomal RNA was removed and libraries for Illumina technology were prepared. Sequencing was done in NovaSeq instrument for 2 x 150 nt. Data were processed using Geneious, pipeline included de novo assembly, contigs search by TBLASTX for plant viruses using a local database. Reads were then mapped back to contigs. A number of viruses were identified in analyzed samples: six viruses and two viroids in grapevines; two viruses in fruit trees; 11 viruses including four novel viruses in ornamentals.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů