Validation of molecular markers for the identification of resistant plants to Hyaloperenospora parasitica in commercial crops of Brassica oleracea L
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F22%3A10175411" target="_blank" >RIV/00027006:_____/22:10175411 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Validation of molecular markers for the identification of resistant plants to Hyaloperenospora parasitica in commercial crops of Brassica oleracea L
Popis výsledku v původním jazyce
Molecular marker technology is integrated into existing plant breeding programs, and resistant genotypes selected using molecular markers can be used for hybridization. This study evaluated the association of the BODM1 molecular marker with resistance to Hyaloperenospora parasitica for the set of 24 (resistant, susceptible) Brassica oleracea genotypes.Using the SSR marker BODM1, the tested samples' observed sections were cloned, sequenced, and then evaluated.The marker did not show length polymorphism between resistant and susceptible varieties. However, sequencing of these samples indicated a 96% variability in the tandem ATC sequence located in the 3rd intron of the glucosinolate pathway of Brassica species, which could be used as a molecular marker in future studies.
Název v anglickém jazyce
Validation of molecular markers for the identification of resistant plants to Hyaloperenospora parasitica in commercial crops of Brassica oleracea L
Popis výsledku anglicky
Molecular marker technology is integrated into existing plant breeding programs, and resistant genotypes selected using molecular markers can be used for hybridization. This study evaluated the association of the BODM1 molecular marker with resistance to Hyaloperenospora parasitica for the set of 24 (resistant, susceptible) Brassica oleracea genotypes.Using the SSR marker BODM1, the tested samples' observed sections were cloned, sequenced, and then evaluated.The marker did not show length polymorphism between resistant and susceptible varieties. However, sequencing of these samples indicated a 96% variability in the tandem ATC sequence located in the 3rd intron of the glucosinolate pathway of Brassica species, which could be used as a molecular marker in future studies.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Návaznosti výsledku
Projekt
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Návaznosti
R - Projekt Ramcoveho programu EK
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů