Development of a PVS3 plant vitrification method for cryopreservation of yacon (Smallanthus sonchifolius) genetic resources

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F22%3A10175738" target="_blank" >RIV/00027006:_____/22:10175738 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Development of a PVS3 plant vitrification method for cryopreservation of yacon (Smallanthus sonchifolius) genetic resources

Popis výsledku v původním jazyce

Cryopreservation is a biotechnological method that plays a vital role invegetatively propagated plant species conservation programs. This method is one ofthe most valuable methods for the long-term conservation of biological materials. It isused as an alternative method to safeguard plant genetic resources currently beingpreserved by conventional methods, such as field collections. The present study aimsat developing an efficient cryopreservation method for the long-term storage of fiveyacon [Smallanthus sonchifolius (Poepp. and Endl.) Robinson] cultivars using PVS3.This vegetatively propagated crop is mainly cultivated for its edible tuberous rootsrich in inulin-type fructooligosaccharides. To carry out the experiments, the plantmaterial was transferred in vitro (surface-sterilized using 70% ethanol for 1 min and2% NaClO for 15 min, rinsed three times in sterile distilled water, and transferred toMS medium). Apical shoot tip (2-3 mm long) comprising the meristematic dome plus2 primordial leaves were then excised from in vitro plantlets. These were then placedin a PVS3 loading solution (20 min); thereafter, they were placed in the PVS3 foreither 30, 45, 60, or 75 min treatment time duration, after which they were thenplaced in liquid nitrogen (LN). After 1hr exposure to LN, they were then thawed andplaced on either MS or MS+1 mg/l BA as recovery media. Parameters such assurvival, regrowth, and signs of morphological abnormalities were assessed in twoweek intervals (up to 8 weeks). The results showed that PVS3 is an effectivecryopreservation method for the long-term conservation of yacon with a 60 mintreatment time duration in combination with MS without 1 mg/l BA as the optimaltreatment, ensuring high survival and regrowth rates ranging from 94% - 80% and 75- 62%, respectively, for the five tested cultivars of yacon. The developed method canensure the safe long-term storage of yacon species currently being conserved byconventional conservation methods.

Název v anglickém jazyce

Development of a PVS3 plant vitrification method for cryopreservation of yacon (Smallanthus sonchifolius) genetic resources

Popis výsledku anglicky

Cryopreservation is a biotechnological method that plays a vital role invegetatively propagated plant species conservation programs. This method is one ofthe most valuable methods for the long-term conservation of biological materials. It isused as an alternative method to safeguard plant genetic resources currently beingpreserved by conventional methods, such as field collections. The present study aimsat developing an efficient cryopreservation method for the long-term storage of fiveyacon [Smallanthus sonchifolius (Poepp. and Endl.) Robinson] cultivars using PVS3.This vegetatively propagated crop is mainly cultivated for its edible tuberous rootsrich in inulin-type fructooligosaccharides. To carry out the experiments, the plantmaterial was transferred in vitro (surface-sterilized using 70% ethanol for 1 min and2% NaClO for 15 min, rinsed three times in sterile distilled water, and transferred toMS medium). Apical shoot tip (2-3 mm long) comprising the meristematic dome plus2 primordial leaves were then excised from in vitro plantlets. These were then placedin a PVS3 loading solution (20 min); thereafter, they were placed in the PVS3 foreither 30, 45, 60, or 75 min treatment time duration, after which they were thenplaced in liquid nitrogen (LN). After 1hr exposure to LN, they were then thawed andplaced on either MS or MS+1 mg/l BA as recovery media. Parameters such assurvival, regrowth, and signs of morphological abnormalities were assessed in twoweek intervals (up to 8 weeks). The results showed that PVS3 is an effectivecryopreservation method for the long-term conservation of yacon with a 60 mintreatment time duration in combination with MS without 1 mg/l BA as the optimaltreatment, ensuring high survival and regrowth rates ranging from 94% - 80% and 75- 62%, respectively, for the five tested cultivars of yacon. The developed method canensure the safe long-term storage of yacon species currently being conserved byconventional conservation methods.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů