Elimination of Black raspberry necrosis virus from raspberry (Rubus idaeus L.) by in vitro cryotherapy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F24%3A10177368" target="_blank" >RIV/00027006:_____/24:10177368 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/24:00601740

Výsledek na webu

<a href="https://www.pubhort.org/ejhs/89/3/16/89_3_16.pdf" target="_blank" >https://www.pubhort.org/ejhs/89/3/16/89_3_16.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/eJHS.2024/016" target="_blank" >10.17660/eJHS.2024/016</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Elimination of Black raspberry necrosis virus from raspberry (Rubus idaeus L.) by in vitro cryotherapy

Popis výsledku v původním jazyce

This study aims to establish a cryopreservation protocol for in vitro grown raspberry (Rubus idaeus L.) without the use of dimethyl sulfoxide (DMSO). The protocol exploits cryotherapy for virus eradication in selected favourable genotypes and will facilitate safe preservation of raspberry genotypes in the Czech national cryobank. Raspberries are highly valuable perennial berries grown in temperate countries including the Czech Republic. Raspberry cultivars and elite breeding lines must be maintained as plant collections either in the field or as in vitro cultures. Both maintenance methods are suboptimal primarily because of the many viral and other pathogens transmitted in raspberry field collections, the industriousness, the maintenance costs, and possible somaclonal variation associated with maintenance of in vitro cultures. For these reasons, cryopreservation is currently considered as a prospective method for the long-term preservation of germplasm. One of the best-performing vitrification procedures for plant cryopreservation is based on DMSO solutions. However, due to the potentially mutagenic effect of DMSO, which is contradictory to the principle of germplasm preservation, we focused on testing a DMSO-free- free procedure using two raspberry varieties. Regrowth rate of the raspberry variety &apos;Tulameen&apos; after application of Plant Vitrification Solution 3 (PVS3) followed by immersion in liquid nitrogen (LN) was 86%. For the raspberry variety Autumn First, the regrowth level was 73%. In vitro shoots of the cryopreserved variety &apos;Tulameen&apos; were multiplied in a standard cultivation medium and RT-PCR screened for eradication of Black raspberry necrosis virus (BRNV). The eradication rate of BRNV from in vitro cultures of &apos;Tulameen&apos; by cryopreservation was 94%. The eradication of BRNV from control samples, not subjected to LN, was not significantly different (92%). It can be concluded that the DMSO-free- free tested method is suitable for efficient cryoconservation. Although the LN phase of the cryoprotocol is not required, the PVS3 protocol alone represents a valuable tool for eradication of BRNV from in vitro cultures of tested raspberry shoots. The suggested protocol will be used for the safe backup of healthy plant material.

Název v anglickém jazyce

Elimination of Black raspberry necrosis virus from raspberry (Rubus idaeus L.) by in vitro cryotherapy

Popis výsledku anglicky

This study aims to establish a cryopreservation protocol for in vitro grown raspberry (Rubus idaeus L.) without the use of dimethyl sulfoxide (DMSO). The protocol exploits cryotherapy for virus eradication in selected favourable genotypes and will facilitate safe preservation of raspberry genotypes in the Czech national cryobank. Raspberries are highly valuable perennial berries grown in temperate countries including the Czech Republic. Raspberry cultivars and elite breeding lines must be maintained as plant collections either in the field or as in vitro cultures. Both maintenance methods are suboptimal primarily because of the many viral and other pathogens transmitted in raspberry field collections, the industriousness, the maintenance costs, and possible somaclonal variation associated with maintenance of in vitro cultures. For these reasons, cryopreservation is currently considered as a prospective method for the long-term preservation of germplasm. One of the best-performing vitrification procedures for plant cryopreservation is based on DMSO solutions. However, due to the potentially mutagenic effect of DMSO, which is contradictory to the principle of germplasm preservation, we focused on testing a DMSO-free- free procedure using two raspberry varieties. Regrowth rate of the raspberry variety &apos;Tulameen&apos; after application of Plant Vitrification Solution 3 (PVS3) followed by immersion in liquid nitrogen (LN) was 86%. For the raspberry variety Autumn First, the regrowth level was 73%. In vitro shoots of the cryopreserved variety &apos;Tulameen&apos; were multiplied in a standard cultivation medium and RT-PCR screened for eradication of Black raspberry necrosis virus (BRNV). The eradication rate of BRNV from in vitro cultures of &apos;Tulameen&apos; by cryopreservation was 94%. The eradication of BRNV from control samples, not subjected to LN, was not significantly different (92%). It can be concluded that the DMSO-free- free tested method is suitable for efficient cryoconservation. Although the LN phase of the cryoprotocol is not required, the PVS3 protocol alone represents a valuable tool for eradication of BRNV from in vitro cultures of tested raspberry shoots. The suggested protocol will be used for the safe backup of healthy plant material.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/TO01000295" target="_blank" >TO01000295: Zdravé ovoce v měnících se klimatických podmínkách: vývoj nových biotechnologických postupů diagnostiky virů, studium vektorů, ozdravování a bezpečného uchovávání jahodníku a maliníku</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

EUROPEAN JOURNAL OF HORTICULTURAL SCIENCE

ISSN

1611-4426

e-ISSN

—

Svazek periodika

89

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

6

Strana od-do

eJHS2024016

Kód UT WoS článku

001332714700002

EID výsledku v databázi Scopus

2-s2.0-85210443526