Enhancing the conservation of garlic (Allium sativum L.) through basal plate cryopreservation and somatic embryogenesis regeneration

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F23%3A10176640" target="_blank" >RIV/00027006:_____/23:10176640 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Enhancing the conservation of garlic (Allium sativum L.) through basal plate cryopreservation and somatic embryogenesis regeneration

Popis výsledku v původním jazyce

This study focused on improving the cryopreservation of garlic when using basal plate. The problem of high infection rate by soil microorganisms was solved using a perchlorate and PPM disinfection scheme for an almost 100% shoot decontamination. The difficulty of excision the apex from such material was solved by dividing the basal plate into two layers and then cutting it into 4-8 pieces 1x1 mm in size. The cryoprotocol was optimized for this size of basal plate sections. The use of a basal plate for garlic cryopreservation has been previously published, but we improved some steps of the cryopreservation procedure to increase the number of regenerating plants. The improvement was in an optimized time in the PVS3 medium and regeneration medium. The highest survival (65%) and regeneration (60%) with countless shoots per basal plate section after cryopreservation was achieved after a 60 min PVS3 treatment time duration (+20 min loading solution). The thawing solution was 0.8 M sucrose dissolved in MS salts, applied for 20 min at 40 oC at the start of thawing. The number of cloves per bulb is essential for the success of cryopreservation. It varies from 3 to 25 for different subgroups. Despite the potential of hundreds of embryos to regenerate by somatic embryogenesis, only a small fraction of a dozen can regenerate into a new plant from one piece of the basal plate, but this is still sufficient for cryopreservation. The use of basal plates has two advantages. First, the plant sources come from harvested garlic bulbs; therefore, the time and labour-consuming in vitro propagation are eschewed before cryopreservation. Secondly is the high occurrence of somatic embryogenesis; many regenerates can be obtained from one small piece of basal plate. The proposed procedure increases the probability of the number of regenerating plants after cryopreservation.

Název v anglickém jazyce

Enhancing the conservation of garlic (Allium sativum L.) through basal plate cryopreservation and somatic embryogenesis regeneration

Popis výsledku anglicky

This study focused on improving the cryopreservation of garlic when using basal plate. The problem of high infection rate by soil microorganisms was solved using a perchlorate and PPM disinfection scheme for an almost 100% shoot decontamination. The difficulty of excision the apex from such material was solved by dividing the basal plate into two layers and then cutting it into 4-8 pieces 1x1 mm in size. The cryoprotocol was optimized for this size of basal plate sections. The use of a basal plate for garlic cryopreservation has been previously published, but we improved some steps of the cryopreservation procedure to increase the number of regenerating plants. The improvement was in an optimized time in the PVS3 medium and regeneration medium. The highest survival (65%) and regeneration (60%) with countless shoots per basal plate section after cryopreservation was achieved after a 60 min PVS3 treatment time duration (+20 min loading solution). The thawing solution was 0.8 M sucrose dissolved in MS salts, applied for 20 min at 40 oC at the start of thawing. The number of cloves per bulb is essential for the success of cryopreservation. It varies from 3 to 25 for different subgroups. Despite the potential of hundreds of embryos to regenerate by somatic embryogenesis, only a small fraction of a dozen can regenerate into a new plant from one piece of the basal plate, but this is still sufficient for cryopreservation. The use of basal plates has two advantages. First, the plant sources come from harvested garlic bulbs; therefore, the time and labour-consuming in vitro propagation are eschewed before cryopreservation. Secondly is the high occurrence of somatic embryogenesis; many regenerates can be obtained from one small piece of basal plate. The proposed procedure increases the probability of the number of regenerating plants after cryopreservation.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/QK1910476" target="_blank" >QK1910476: Zvýšení výnosů a kvality produkce česneku výběrem suchovzdorných a chladuvzdorných klonů na základě molekulárně genetické analýzy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů