Application of differential thermal analysis for the improved regeneration after cryopreservation of vegetatively-propagated plants

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F19%3A00005116" target="_blank" >RIV/00027006:_____/19:00005116 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.17660/ActaHortic.2019.1234.7" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2019.1234.7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/ActaHortic.2019.1234.7" target="_blank" >10.17660/ActaHortic.2019.1234.7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Application of differential thermal analysis for the improved regeneration after cryopreservation of vegetatively-propagated plants

Popis výsledku v původním jazyce

Three critical key parameters for cryoprotocol improvement resulting in increasing the regeneration rate after cryopreservation can be determined by thermal analysis: 1) the content of frozen water. The boundary content of frozen water affected by dehydration over desiccants or a mixture of cryoprotectants can be determined by differential scanning calorimetry; in the case of cryopreservation of garlic shoot tips, by dehydration in different concentrations of Plant Vitrification Solution No 3 (PVS3). Particular concentrations of cryoprotectants can regulate the final frozen water content. Although the regeneration of garlic shoot tips after their exposure to diluted PVS3 (i.e., sucrose/glycerol components 45/45) was similar to PVS3 (sucrose/glycerol 50/50), the use of less concentrated PVS3 reduces the risk of dehydration damage before ultra-low temperature application; 2) the content of unfreezable water. The intersection of the content of frozen water and the absolute content of water can be determined gravimetrically in shoot tips at different levels of dehydration and the content of unfreezable water derived. The unfreezable water content can be the limit of the minimum water content below which the shoot tips cannot survive dehydration; and 3) glass transition temperature. Determination of the glass transition temperature is important for long-term cryopreservation without undesirable alterations to the sample. A pure cryoprotectant, added usually in an excessive amount, has a constant Tg; whereas, the Tg of shoot tips increases as the content of water removed with the cryoprotective solution decreases. The highest recovery level after cryopreservation treatment should be achieved at the highest obtained sample glass transition temperature. The three above-mentioned parameters define the threshold dehydration level of shoot tips important for a high regeneration rate after cryopreservation.

Název v anglickém jazyce

Application of differential thermal analysis for the improved regeneration after cryopreservation of vegetatively-propagated plants

Popis výsledku anglicky

Three critical key parameters for cryoprotocol improvement resulting in increasing the regeneration rate after cryopreservation can be determined by thermal analysis: 1) the content of frozen water. The boundary content of frozen water affected by dehydration over desiccants or a mixture of cryoprotectants can be determined by differential scanning calorimetry; in the case of cryopreservation of garlic shoot tips, by dehydration in different concentrations of Plant Vitrification Solution No 3 (PVS3). Particular concentrations of cryoprotectants can regulate the final frozen water content. Although the regeneration of garlic shoot tips after their exposure to diluted PVS3 (i.e., sucrose/glycerol components 45/45) was similar to PVS3 (sucrose/glycerol 50/50), the use of less concentrated PVS3 reduces the risk of dehydration damage before ultra-low temperature application; 2) the content of unfreezable water. The intersection of the content of frozen water and the absolute content of water can be determined gravimetrically in shoot tips at different levels of dehydration and the content of unfreezable water derived. The unfreezable water content can be the limit of the minimum water content below which the shoot tips cannot survive dehydration; and 3) glass transition temperature. Determination of the glass transition temperature is important for long-term cryopreservation without undesirable alterations to the sample. A pure cryoprotectant, added usually in an excessive amount, has a constant Tg; whereas, the Tg of shoot tips increases as the content of water removed with the cryoprotective solution decreases. The highest recovery level after cryopreservation treatment should be achieved at the highest obtained sample glass transition temperature. The three above-mentioned parameters define the threshold dehydration level of shoot tips important for a high regeneration rate after cryopreservation.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40401 - Agricultural biotechnology and food biotechnology

Projekt

<a href="/cs/project/QJ1630301" target="_blank" >QJ1630301: Tvorba nových systémů biotechnologických opatření pro zachování a rozvoj biodiverzity zemědělských plodin a lesních dřevin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the III International Symposium on Plant Cryopreservation

ISBN

978-94-62612-31-0

ISSN

0567-7572

e-ISSN

—

Počet stran výsledku

8

Strana od-do

57-64

Název nakladatele

International Society for Horticultural Science (ISHS)

Místo vydání

Belgium

Místo konání akce

Bangkok, Thailand

Datum konání akce

1. 1. 2018

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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