Dehydration status of ABA-treated and cold-acclimated Hypericum perforatum L. shoot tips subjected to cryopreservation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F11%3A00001873" target="_blank" >RIV/00027006:_____/11:00001873 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.tca.2011.07.022" target="_blank" >http://dx.doi.org/10.1016/j.tca.2011.07.022</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.tca.2011.07.022" target="_blank" >10.1016/j.tca.2011.07.022</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Dehydration status of ABA-treated and cold-acclimated Hypericum perforatum L. shoot tips subjected to cryopreservation

Popis výsledku v původním jazyce

To improve the vitrification protocol for Hypericum perforatum shoot tips, calorimetric measurements of the intensity of thermal transitions in the samples during warming using differential scanning calorimetry were assessed. Comparison of ABA treatmentand cold-acclimation revealed higher content of crystallizable water in the latter. Regeneration capacity of cryopreserved H. perforatum shoot tips increased progressively with the exposure time in PVS3 cryoprotection mixture reaching the maximum between59.3 and 71.3% after 180 min when the proportion of freezable water ranged between 0.0 and 0.4% of total water content respectively. Glass transitions were observed in all samples dehydrated with PVS3 when freezable water was present. However, the decreasing recovery of H. perforatum shoot tips after prolonged PVS3 dehydration (without LN storage) indicated some injuries of meristematic tissue induced by PVS3 dehydration rather than ice nucleation and resulted from genotype-dependent to

Název v anglickém jazyce

Dehydration status of ABA-treated and cold-acclimated Hypericum perforatum L. shoot tips subjected to cryopreservation

Popis výsledku anglicky

To improve the vitrification protocol for Hypericum perforatum shoot tips, calorimetric measurements of the intensity of thermal transitions in the samples during warming using differential scanning calorimetry were assessed. Comparison of ABA treatmentand cold-acclimation revealed higher content of crystallizable water in the latter. Regeneration capacity of cryopreserved H. perforatum shoot tips increased progressively with the exposure time in PVS3 cryoprotection mixture reaching the maximum between59.3 and 71.3% after 180 min when the proportion of freezable water ranged between 0.0 and 0.4% of total water content respectively. Glass transitions were observed in all samples dehydrated with PVS3 when freezable water was present. However, the decreasing recovery of H. perforatum shoot tips after prolonged PVS3 dehydration (without LN storage) indicated some injuries of meristematic tissue induced by PVS3 dehydration rather than ice nucleation and resulted from genotype-dependent to

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Thermochimica Acta

ISSN

0040-6031

e-ISSN

—

Svazek periodika

525

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

62-70

Kód UT WoS článku

000296824400009

EID výsledku v databázi Scopus

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