Preparation of Shoot Tips by Sucrose and Dehydration Pre-Treatment of Ullucus tuberosus Cal. for Cryopreservation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F11%3A00002048" target="_blank" >RIV/00027006:_____/11:00002048 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41610/11:53903

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Preparation of Shoot Tips by Sucrose and Dehydration Pre-Treatment of Ullucus tuberosus Cal. for Cryopreservation

Popis výsledku v původním jazyce

Ullucus tuberosus (ulluco) is a vegetatively propagated tuber crop of the Andean region. An method for cryopreservation of ulluco was tested with the aim of long term storage of U. tuberosus genetic resources. The effect of a sucrose pre-treatment improved the tolerance of shoot tips towards PVS3 dehydration. After two hours of desiccation over silicagel, the regeneration of thawed shoot tips from liquid nitrogen reached only 10%. This desiccation decreased the ice nucleation (taking place at -36°C) by6°C which could help to narrow down the time that lethal ice nucleation can take place before glass formation. PVS3 pre-treatment decreases the percentage of crystallizable water to less than 2% of water. The combination of sucrose pre-treatment and 2 hdesiccation over silica gel allowed 10% of the shoot tips to survive and regrow following cryopreservation. In the case where shoot tips were treated with PVS3 for 1.5 h, regeneration percentage reached 52.5%.

Název v anglickém jazyce

Preparation of Shoot Tips by Sucrose and Dehydration Pre-Treatment of Ullucus tuberosus Cal. for Cryopreservation

Popis výsledku anglicky

Ullucus tuberosus (ulluco) is a vegetatively propagated tuber crop of the Andean region. An method for cryopreservation of ulluco was tested with the aim of long term storage of U. tuberosus genetic resources. The effect of a sucrose pre-treatment improved the tolerance of shoot tips towards PVS3 dehydration. After two hours of desiccation over silicagel, the regeneration of thawed shoot tips from liquid nitrogen reached only 10%. This desiccation decreased the ice nucleation (taking place at -36°C) by6°C which could help to narrow down the time that lethal ice nucleation can take place before glass formation. PVS3 pre-treatment decreases the percentage of crystallizable water to less than 2% of water. The combination of sucrose pre-treatment and 2 hdesiccation over silica gel allowed 10% of the shoot tips to survive and regrow following cryopreservation. In the case where shoot tips were treated with PVS3 for 1.5 h, regeneration percentage reached 52.5%.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Horticulturae

ISSN

0567-7572

e-ISSN

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Svazek periodika

neuveden

Číslo periodika v rámci svazku

908

Stát vydavatele periodika

BE - Belgické království

Počet stran výsledku

8

Strana od-do

331-338

Kód UT WoS článku

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EID výsledku v databázi Scopus

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