Prospects of using surfactants for cryopreservation of peach shoot tips

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F25%3A10178100" target="_blank" >RIV/00027006:_____/25:10178100 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Prospects of using surfactants for cryopreservation of peach shoot tips

Popis výsledku v původním jazyce

Cryopreservation is a critical method for preserving plant genetic resources, particularly forspecies such as peach (Prunus persica), which cannot be effectively stored as seeds.However, the sensitivity of peach shoot tips to cryogenic stress often results in low viabilityand poor regrowth due to dehydration, oxidative stress, and cellular damage. This studyevaluates the potential of the surfactant Pluronic F-68 to enhance the viability and regrowthof cryopreserved shoot tips from four peach genotypes: Flame Prince, Vate 1543,Guglielmina, and Nectared 4. A droplet-vitrification approach was used, incorporatingPluronic F-68 at various stages of the process, including loading, vitrification, and post-thawrecovery solutions. Long-term exposure to PVS3 in the control group (without surfactant)significantly reduced viability, highlighting the cytotoxic effects of vitrification solutions.Compared to the control, the inclusion of Pluronic F-68 resulted in a twofold increase inshoot tip regrowth. The improved outcomes suggest that the surfactant helps stabilizemembranes, reduce osmotic stress, and mitigate dehydration damage during cryopreservation.These findings underscore the surfactant&apos;s potential as a valuable additive for optimizingcryopreservation protocols, facilitating the efficient conservation of peach germplasm forbreeding and preservation programs.

Název v anglickém jazyce

Prospects of using surfactants for cryopreservation of peach shoot tips

Popis výsledku anglicky

Cryopreservation is a critical method for preserving plant genetic resources, particularly forspecies such as peach (Prunus persica), which cannot be effectively stored as seeds.However, the sensitivity of peach shoot tips to cryogenic stress often results in low viabilityand poor regrowth due to dehydration, oxidative stress, and cellular damage. This studyevaluates the potential of the surfactant Pluronic F-68 to enhance the viability and regrowthof cryopreserved shoot tips from four peach genotypes: Flame Prince, Vate 1543,Guglielmina, and Nectared 4. A droplet-vitrification approach was used, incorporatingPluronic F-68 at various stages of the process, including loading, vitrification, and post-thawrecovery solutions. Long-term exposure to PVS3 in the control group (without surfactant)significantly reduced viability, highlighting the cytotoxic effects of vitrification solutions.Compared to the control, the inclusion of Pluronic F-68 resulted in a twofold increase inshoot tip regrowth. The improved outcomes suggest that the surfactant helps stabilizemembranes, reduce osmotic stress, and mitigate dehydration damage during cryopreservation.These findings underscore the surfactant&apos;s potential as a valuable additive for optimizingcryopreservation protocols, facilitating the efficient conservation of peach germplasm forbreeding and preservation programs.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

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Návaznosti

R - Projekt Ramcoveho programu EK

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů