Utilizing Alginate Hydrogels to Enhance Cryopreservation Efficiency in Peach (Prunus persica) Shoot Tips

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F25%3A10179639" target="_blank" >RIV/00027006:_____/25:10179639 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Utilizing Alginate Hydrogels to Enhance Cryopreservation Efficiency in Peach (Prunus persica) Shoot Tips

Popis výsledku v původním jazyce

The long-term conservation of Prunus persica (peach), a fruit crop of major commercial and genetic importance, is hindered by the species’ limited response to conventional cryopreservation methods. Recalcitrance to dehydration and cryoprotectant toxicity often leads to low survival and regrowth rates, posing significant challenges for the preservation of clonal germplasm. This study explores a novel cryopreservation approach for peach shoot tips using a modified D-cryoplate technique, enhanced by the inclusion of Pluronic F-68, a non-ionic surfactant known for its membrane-stabilizing properties. In vitro-grown shoot tips (2–3 mm) from six peach genotypes were precultured in stepwise sucrose concentrations and then embedded in a thin layer of sodium alginate hydrogel directly on aluminium strips. This hydrogel matrix acted not only as a mechanical stabilizer during cryoprotectant exposure and liquid nitrogen immersion but also as a modulator of water loss, enabling more controlled and uniform dehydration. Cryoprotection was performed using a loading solution and PVS3, both with and without Pluronic F-68. Following rapid cooling in liquid nitrogen and subsequent thawing, shoot tips were unloaded and transferred to recovery medium for regeneration assessment. The integration of alginate hydrogels significantly improved shoot tip regrowth, particularly when combined with Pluronic F-68. The highest regeneration rates ‒ up to twice that of untreated controls‒were observed in samples receiving both treatments. The D-cryoplate platform, with its fixed physical support and defined dehydration environment, further contributed to improved survival by minimizing tissue handling and optimizing vitrification dynamics. These findings confirm the synergistic benefits of using alginate hydrogels and surfactants within structured cryopreservation systems. Příspěvek v Book of Abstracts, 61st meeting Society for Low Temperature Biology

Název v anglickém jazyce

Utilizing Alginate Hydrogels to Enhance Cryopreservation Efficiency in Peach (Prunus persica) Shoot Tips

Popis výsledku anglicky

The long-term conservation of Prunus persica (peach), a fruit crop of major commercial and genetic importance, is hindered by the species’ limited response to conventional cryopreservation methods. Recalcitrance to dehydration and cryoprotectant toxicity often leads to low survival and regrowth rates, posing significant challenges for the preservation of clonal germplasm. This study explores a novel cryopreservation approach for peach shoot tips using a modified D-cryoplate technique, enhanced by the inclusion of Pluronic F-68, a non-ionic surfactant known for its membrane-stabilizing properties. In vitro-grown shoot tips (2–3 mm) from six peach genotypes were precultured in stepwise sucrose concentrations and then embedded in a thin layer of sodium alginate hydrogel directly on aluminium strips. This hydrogel matrix acted not only as a mechanical stabilizer during cryoprotectant exposure and liquid nitrogen immersion but also as a modulator of water loss, enabling more controlled and uniform dehydration. Cryoprotection was performed using a loading solution and PVS3, both with and without Pluronic F-68. Following rapid cooling in liquid nitrogen and subsequent thawing, shoot tips were unloaded and transferred to recovery medium for regeneration assessment. The integration of alginate hydrogels significantly improved shoot tip regrowth, particularly when combined with Pluronic F-68. The highest regeneration rates ‒ up to twice that of untreated controls‒were observed in samples receiving both treatments. The D-cryoplate platform, with its fixed physical support and defined dehydration environment, further contributed to improved survival by minimizing tissue handling and optimizing vitrification dynamics. These findings confirm the synergistic benefits of using alginate hydrogels and surfactants within structured cryopreservation systems. Příspěvek v Book of Abstracts, 61st meeting Society for Low Temperature Biology

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů