Effect of Explant Physiology and Media Composition on Callogenesis of Vitellaria paradoxa Leaf Explants

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F25%3A10179172" target="_blank" >RIV/00027006:_____/25:10179172 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/2311-7524/11/9/1127/pdf?version=1758505536" target="_blank" >https://www.mdpi.com/2311-7524/11/9/1127/pdf?version=1758505536</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/horticulturae11091127" target="_blank" >10.3390/horticulturae11091127</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of Explant Physiology and Media Composition on Callogenesis of Vitellaria paradoxa Leaf Explants

Popis výsledku v původním jazyce

Vitellaria paradoxa (shea tree) is an economically and medicinally important species indigenous to sub-Saharan Africa. Although the species holds substantial value, domestication efforts have been constrained, primarily due to the absence of efficient propagation alternatives, especially for the East African subspecies (V. paradoxa subsp. nilotica) which remains understudied in tissue culture research. This study investigated the influence of leaf explant developmental stage and media composition on callogenesis and embryogenic potential in V. paradoxa subsp. nilotica. Thus, leaf explants from six distinct growth stages were cultured on Murashige and Skoog (MS) media supplemented with various concentrations of 2,4-D, TDZ, NAA, and BAP. Callogenesis was significantly influenced by explant age, media strength, and specific PGR combinations. Results revealed that explants from Stage III (11-15 days) and Stage IV (16-20 days) exhibited the highest callus induction rates (up to 100%), particularly on half-strength MS media containing 2.0 mg/L 2,4-D and 0.5-1.0 mg/L TDZ. Histological analysis suggests that varying responses at the different stages relate to chloroplast distribution, trichome density/orientation, and vascular tissue maturity. Pro-embryogenic structures were successfully induced, representing a developmental milestone with strong prospects for advanced stages of differentiation. The findings also emphasize the importance of explant physiology and media formulation in developing regeneration protocols for V. paradoxa from leaf explants.

Název v anglickém jazyce

Effect of Explant Physiology and Media Composition on Callogenesis of Vitellaria paradoxa Leaf Explants

Popis výsledku anglicky

Vitellaria paradoxa (shea tree) is an economically and medicinally important species indigenous to sub-Saharan Africa. Although the species holds substantial value, domestication efforts have been constrained, primarily due to the absence of efficient propagation alternatives, especially for the East African subspecies (V. paradoxa subsp. nilotica) which remains understudied in tissue culture research. This study investigated the influence of leaf explant developmental stage and media composition on callogenesis and embryogenic potential in V. paradoxa subsp. nilotica. Thus, leaf explants from six distinct growth stages were cultured on Murashige and Skoog (MS) media supplemented with various concentrations of 2,4-D, TDZ, NAA, and BAP. Callogenesis was significantly influenced by explant age, media strength, and specific PGR combinations. Results revealed that explants from Stage III (11-15 days) and Stage IV (16-20 days) exhibited the highest callus induction rates (up to 100%), particularly on half-strength MS media containing 2.0 mg/L 2,4-D and 0.5-1.0 mg/L TDZ. Histological analysis suggests that varying responses at the different stages relate to chloroplast distribution, trichome density/orientation, and vascular tissue maturity. Pro-embryogenic structures were successfully induced, representing a developmental milestone with strong prospects for advanced stages of differentiation. The findings also emphasize the importance of explant physiology and media formulation in developing regeneration protocols for V. paradoxa from leaf explants.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Horticulturae

ISSN

2311-7524

e-ISSN

2311-7524

Svazek periodika

11

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

13

Strana od-do

1127

Kód UT WoS článku

001580026900001

EID výsledku v databázi Scopus

2-s2.0-105017396132