Vliv osmotickéhio stresu na toleranci k dehydrataci a kryoprezervace vzrostných vrcholů Solanum tuberosum shoot tips.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F99%3A%230000029" target="_blank" >RIV/00027006:_____/99:#0000029 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of osmotic stress on the dehydration tolerance and cryopreservation of Solanum tuberosum shoot tips.

Popis výsledku v původním jazyce

"Four different preculture treatments were tested for their ability to enhance survival of potato shoot tips (cv. Desirée) after both dehydration and cryopreservation. The encapsulation/dehydration method and rapid freezing were used. Further, accumulation of sucrose, glucose, fructose, glycerol and proline in the apices during the treatments was measured. Very high internal sucrose concentrations (up to 27.8 % of dry mass) was essential to reach high survival rates after cryopreservation, but not afterdehydration only. The highest survival rate (78.8%) was obtained when osmotic stress was induced by adding 2 M sucrose solution to donor plants for 5 days, followed by 0.7 M sucrose preculture of isolated shoot tips for 1 day. The results demonstrate that drought hardening would be able to replace the effect of low temperatures in such plants, which are not able to be cold hardened.

Název v anglickém jazyce

Effect of osmotic stress on the dehydration tolerance and cryopreservation of Solanum tuberosum shoot tips.

Popis výsledku anglicky

"Four different preculture treatments were tested for their ability to enhance survival of potato shoot tips (cv. Desirée) after both dehydration and cryopreservation. The encapsulation/dehydration method and rapid freezing were used. Further, accumulation of sucrose, glucose, fructose, glycerol and proline in the apices during the treatments was measured. Very high internal sucrose concentrations (up to 27.8 % of dry mass) was essential to reach high survival rates after cryopreservation, but not afterdehydration only. The highest survival rate (78.8%) was obtained when osmotic stress was induced by adding 2 M sucrose solution to donor plants for 5 days, followed by 0.7 M sucrose preculture of isolated shoot tips for 1 day. The results demonstrate that drought hardening would be able to replace the effect of low temperatures in such plants, which are not able to be cold hardened.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

<a href="/cs/project/GA522%2F98%2F0861" target="_blank" >GA522/98/0861: Kryoprezervace rostlin vitrifikací - metoda zamezující tvorbě ledových krystalů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

1999

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cryo-Letters

ISSN

0143-2044

e-ISSN

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Svazek periodika

20

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

339-346

Kód UT WoS článku

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EID výsledku v databázi Scopus

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