Vliv kofeinu na meiotické zrání oocytů prasat

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F04%3A00010980" target="_blank" >RIV/00027014:_____/04:00010980 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of caffeine on meiotic maturation of porcine oocytes

Popis výsledku v původním jazyce

This study was conducted to evaluate the effect of caffeine on the meiotic maturation of porcine oocytes. Caffeine inhibited the meiotic resumption of pig oocytes effectively after 24 h of culture, and 95.5 % of oocytes were arrested at the germinal vesicle (GV) stage (control 17.8 %, P&lt;0.05). Caffeine also affected embryonic development. The ability of caffeine-treated oocytes to develop to blastocysts after artificial activation was lower than that of the control (5.5 % vs 9.1%, P &lt; 0.05). Caffeine treatment significantly increased cAMP levels in the oocytes after 24 h of culture, while both Cdc2 kinase and MAP kinase activation were inhibited in the oocytes. These results suggest that caffeine, similarly to other purine derivatives, prolongs the meiotic arrest of porcine oocytes at the GV stage, perhaps by its action of increasing the cAMP level and by the suppression of Cdc2 kinase and MAP kinase activities in the oocytes.

Název v anglickém jazyce

Effect of caffeine on meiotic maturation of porcine oocytes

Popis výsledku anglicky

This study was conducted to evaluate the effect of caffeine on the meiotic maturation of porcine oocytes. Caffeine inhibited the meiotic resumption of pig oocytes effectively after 24 h of culture, and 95.5 % of oocytes were arrested at the germinal vesicle (GV) stage (control 17.8 %, P&lt;0.05). Caffeine also affected embryonic development. The ability of caffeine-treated oocytes to develop to blastocysts after artificial activation was lower than that of the control (5.5 % vs 9.1%, P &lt; 0.05). Caffeine treatment significantly increased cAMP levels in the oocytes after 24 h of culture, while both Cdc2 kinase and MAP kinase activation were inhibited in the oocytes. These results suggest that caffeine, similarly to other purine derivatives, prolongs the meiotic arrest of porcine oocytes at the GV stage, perhaps by its action of increasing the cAMP level and by the suppression of Cdc2 kinase and MAP kinase activities in the oocytes.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA524%2F03%2F0927" target="_blank" >GA524/03/0927: Zvýšení vývojové schopností embryí prasete produkovaných in vitro</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Zygote

ISSN

0967-1994

e-ISSN

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Svazek periodika

12

Číslo periodika v rámci svazku

February

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

31-38

Kód UT WoS článku

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EID výsledku v databázi Scopus

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