Effects of cell cycle dependent kinases inhibitor on nuclear and cytoplasmic maturation of porcine oocytes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F01%3A00000097" target="_blank" >RIV/00027014:_____/01:00000097 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effects of cell cycle dependent kinases inhibitor on nuclear and cytoplasmic maturation of porcine oocytes

Popis výsledku v původním jazyce

The aims of this study were to assess the effectiveness of roscovitine a potent inhibitor of cell cyclin kinases to prevent meiotic resumption in porcine oocytes and to test the subsequent fertilisability and developmental competence of these oocytes. Roscovitine blocked porcine oocytes at the GV stage during 22-44 hr of culture. This effect was dose-dependent and a concentration of 25 M was sufficient to prevent meiotic resumption in 92?5% of the oocytes after 22 hr in the presence of EGF and FSH.F andFSH. Cumulus expansion was also inhibited under these conditions. The histone H1 kinase activity in oocytes was inhibited in a dose-dependent way and maintained at a basal level with 25 M of roscovitine. Synthesis of proteins of 29 47 and 79 k normallysynthesized during maturation was inhibited too. All these effects were fully reversible.

Název v anglickém jazyce

Effects of cell cycle dependent kinases inhibitor on nuclear and cytoplasmic maturation of porcine oocytes

Popis výsledku anglicky

The aims of this study were to assess the effectiveness of roscovitine a potent inhibitor of cell cyclin kinases to prevent meiotic resumption in porcine oocytes and to test the subsequent fertilisability and developmental competence of these oocytes. Roscovitine blocked porcine oocytes at the GV stage during 22-44 hr of culture. This effect was dose-dependent and a concentration of 25 M was sufficient to prevent meiotic resumption in 92?5% of the oocytes after 22 hr in the presence of EGF and FSH.F andFSH. Cumulus expansion was also inhibited under these conditions. The histone H1 kinase activity in oocytes was inhibited in a dose-dependent way and maintained at a basal level with 25 M of roscovitine. Synthesis of proteins of 29 47 and 79 k normallysynthesized during maturation was inhibited too. All these effects were fully reversible.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

<a href="/cs/project/GV524%2F96%2FK162" target="_blank" >GV524/96/K162: Molekulární a buněčné základy oogeneze oplození a embryonálního vývoje savců</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Reproduction and Development

ISSN

1040-452X

e-ISSN

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Svazek periodika

60

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

65-73

Kód UT WoS článku

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EID výsledku v databázi Scopus

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