Physiological production of hydrogen sulfide is involved in porcine oocytes maturing in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F15%3A69195" target="_blank" >RIV/60460709:41210/15:69195 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.niox.2015.02.147" target="_blank" >http://dx.doi.org/10.1016/j.niox.2015.02.147</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.niox.2015.02.147" target="_blank" >10.1016/j.niox.2015.02.147</a>

Jazyk výsledku

čeština

Název v původním jazyce

Physiological production of hydrogen sulfide is involved in porcine oocytes maturing in vitro

Popis výsledku v původním jazyce

Oocyte meiotic maturation is indispensable process of female gametogenesis. Hereby matured oocyte is predestined for fertilisation and subsequent embryonic development. The running of meiotic maturation is a result of activity of key factors – M-phase/Maturation Promoting Factor (MPF) and Mitogen Activated Protein Kinase(MAPK). Based on present knowledge, we hypothesise physiological action of hydrogen sulfide produced by CBS, CTH and 3-MPST, hydrogen sulfide releasing enzymes, during in vitro oocyte maturation.Oocytes were in vitro matured in modified M199 culture medium for 48 hrs in 39°C and 5% CO2. Consequently, parthenogenic activation of Na2S-treated oocytes by calcium ionophore A23185 was performed for evaluation of developmental competence. Our results demostrate the presence of mRNA CBS, CTH and 3-MPST CBS, CTH and 3-MPST themselves in porcine oocytes. The inhibition of present enzymes causes the deceleration of meiotic maturation and failure in achievement of fertilizing ability in a regula

Název v anglickém jazyce

Physiological production of hydrogen sulfide is involved in porcine oocytes maturing in vitro

Popis výsledku anglicky

Oocyte meiotic maturation is indispensable process of female gametogenesis. Hereby matured oocyte is predestined for fertilisation and subsequent embryonic development. The running of meiotic maturation is a result of activity of key factors – M-phase/Maturation Promoting Factor (MPF) and Mitogen Activated Protein Kinase(MAPK). Based on present knowledge, we hypothesise physiological action of hydrogen sulfide produced by CBS, CTH and 3-MPST, hydrogen sulfide releasing enzymes, during in vitro oocyte maturation.Oocytes were in vitro matured in modified M199 culture medium for 48 hrs in 39°C and 5% CO2. Consequently, parthenogenic activation of Na2S-treated oocytes by calcium ionophore A23185 was performed for evaluation of developmental competence. Our results demostrate the presence of mRNA CBS, CTH and 3-MPST CBS, CTH and 3-MPST themselves in porcine oocytes. The inhibition of present enzymes causes the deceleration of meiotic maturation and failure in achievement of fertilizing ability in a regula

Druh

D - Stať ve sborníku

CEP obor

GG - Chov hospodářských zvířat

OECD FORD obor

—

Projekt

<a href="/cs/project/QJ1510138" target="_blank" >QJ1510138: Inovace biotechnologií v reprodukci hospodářských zvířat</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

NITRIC OXIDE-BIOLOGY AND CHEMISTRY

ISBN

—

ISSN

1089-8603

e-ISSN

—

Počet stran výsledku

2

Strana od-do

59-60

Název nakladatele

ACADEMIC PRESS INC ELSEVIER SCIENCE

Místo vydání

SAN DIEGO, USA

Místo konání akce

Athens, GREECE

Datum konání akce

3. 5. 2015

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000353313900138