Aktivita telomerázy v prasečích granulosních buňkách proliferujících a diferencujících in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F08%3A%230000796" target="_blank" >RIV/00027014:_____/08:#0000796 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Telomerase activity in pig granulosa cells proliferating and differentiating in vitro

Popis výsledku v původním jazyce

The present study provides the evidence that pig granulosa cells originating from small (SF) and large (LF) ovarian follicles differ in proliferative and differentiative capacities in vitro. SF showed significantly higher proliferation potential in vitro, LF showed significantly advanced steroidogenic functions. Any substantial difference in average telomerase activity between freshly isolated SF and LF was not detected. When cultured, both SF and LF were responsive to EGF and FSH treatment and expressed absolutely higher levels of telomerase activity in comparison to freshly isolated cells. Except of basic and EGF conditions at a 24 h interval, no significantly different levels of telomerase activity between the SF and LF at an interval of 24 and 48 hof in vitro culture were observed. The activity of telomerase in pig ovarian follicle development may not be linked only to granulosa cell proliferation but some regulatory mechanisms of differentiation might be involved, too.

Název v anglickém jazyce

Telomerase activity in pig granulosa cells proliferating and differentiating in vitro

Popis výsledku anglicky

The present study provides the evidence that pig granulosa cells originating from small (SF) and large (LF) ovarian follicles differ in proliferative and differentiative capacities in vitro. SF showed significantly higher proliferation potential in vitro, LF showed significantly advanced steroidogenic functions. Any substantial difference in average telomerase activity between freshly isolated SF and LF was not detected. When cultured, both SF and LF were responsive to EGF and FSH treatment and expressed absolutely higher levels of telomerase activity in comparison to freshly isolated cells. Except of basic and EGF conditions at a 24 h interval, no significantly different levels of telomerase activity between the SF and LF at an interval of 24 and 48 hof in vitro culture were observed. The activity of telomerase in pig ovarian follicle development may not be linked only to granulosa cell proliferation but some regulatory mechanisms of differentiation might be involved, too.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA523%2F05%2F2062" target="_blank" >GA523/05/2062: Vývojový potenciál a molekulární diferenciace folikulárních granulózních buněk prasete in vitro</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Animal Reproduction Science

ISSN

0378-4320

e-ISSN

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Svazek periodika

104

Číslo periodika v rámci svazku

2-4

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

15

Strana od-do

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Kód UT WoS článku

000253599900016

EID výsledku v databázi Scopus

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