Effects of cycloheximide or 6-dimethyl aminopurine on the parthenogenetic activation of pig oocytes using pulsatile treatment with nitric oxide donor

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F09%3A%230001015" target="_blank" >RIV/00027014:_____/09:#0001015 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/09:31054

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effects of cycloheximide or 6-dimethyl aminopurine on the parthenogenetic activation of pig oocytes using pulsatile treatment with nitric oxide donor

Popis výsledku v původním jazyce

Pig oocytes matured in vitro were parthenogenetically activated using nitric oxide donor (SNAP). Continuous treatment successfully activated the oocytes only after more than 12 hours of exposure. Pulsatile treatments were more efficient with regard to the activation rate. The development was very limited after continuous treatment with SNAP. A significantly higher proportion of developing embryos was observed after the pulsatile treatment. However, this developmental rate was significantly lower than the development induced by treatment with calcium ionophore. After combination pulsatile treatment with the effect of protein kinase inhibitor 6-dimethyl aminopurine (6 DMAP) or with the inhibitor of protein synthesis cycloheximide (CHX) a significant increase in the activation rate when compared to the respective pulsatile treatment was observed. However, the development of parthenogenetic embryos was not enhanced when the pulsatile treatment was combined with 6-DMAP or with CHX..

Název v anglickém jazyce

Effects of cycloheximide or 6-dimethyl aminopurine on the parthenogenetic activation of pig oocytes using pulsatile treatment with nitric oxide donor

Popis výsledku anglicky

Pig oocytes matured in vitro were parthenogenetically activated using nitric oxide donor (SNAP). Continuous treatment successfully activated the oocytes only after more than 12 hours of exposure. Pulsatile treatments were more efficient with regard to the activation rate. The development was very limited after continuous treatment with SNAP. A significantly higher proportion of developing embryos was observed after the pulsatile treatment. However, this developmental rate was significantly lower than the development induced by treatment with calcium ionophore. After combination pulsatile treatment with the effect of protein kinase inhibitor 6-dimethyl aminopurine (6 DMAP) or with the inhibitor of protein synthesis cycloheximide (CHX) a significant increase in the activation rate when compared to the respective pulsatile treatment was observed. However, the development of parthenogenetic embryos was not enhanced when the pulsatile treatment was combined with 6-DMAP or with CHX..

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA523%2F06%2F0295" target="_blank" >GA523/06/0295: Aktivace prasečích oocytů donory oxidu dusnatého</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Czech Journal of Animal Science

ISSN

1212-1819

e-ISSN

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Svazek periodika

54

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

14

Strana od-do

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Kód UT WoS článku

000268207300001

EID výsledku v databázi Scopus

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