Parthenogenetic activation of pig oocytes using pulsatile treatment with a nitric oxide donor

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F10%3A48294" target="_blank" >RIV/60460709:41210/10:48294 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378041:_____/10:00343568 RIV/00027014:_____/10:#0001204

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

čeština

Název v původním jazyce

Parthenogenetic activation of pig oocytes using pulsatile treatment with a nitric oxide donor

Popis výsledku v původním jazyce

The nitric oxide donor (+)S-nitroso-N-acetylpenicillamine (SNAP) is capable of inducing parthenogenetic activation in pig oocytes. Repeated short-term treatment with 2 mM SNAP increased the activation rates. With regard to the activation rate, 12 repeated treatments lasting 10 min each were found to be the most efficient regimen (63.3%). The continuous exposure to the NO donor for the same overall time induced parthenogenetic activation in 12.5% oocytes (2-h) continuous treatment with 2 mM SNAP). The development of parthenogenetic embryos increased after repeated short-term treatment with SNAP. After continuous treatment with 2 mM SNAP for 10 h, only 6.7% of the oocytes cleaved, and none developed beyond the 4-cell stage. Thirty-minute treatment repeated four times with 2 mM SNAP induced cleavage in 37.5% of the oocytes, 18.3% developed to the morula stage, and 6.7% reached the blastocyst stage. It is concluded that pulsatile treatment can improve parthenogenetic activation rate.

Název v anglickém jazyce

Parthenogenetic activation of pig oocytes using pulsatile treatment with a nitric oxide donor

Popis výsledku anglicky

The nitric oxide donor (+)S-nitroso-N-acetylpenicillamine (SNAP) is capable of inducing parthenogenetic activation in pig oocytes. Repeated short-term treatment with 2 mM SNAP increased the activation rates. With regard to the activation rate, 12 repeated treatments lasting 10 min each were found to be the most efficient regimen (63.3%). The continuous exposure to the NO donor for the same overall time induced parthenogenetic activation in 12.5% oocytes (2-h) continuous treatment with 2 mM SNAP). The development of parthenogenetic embryos increased after repeated short-term treatment with SNAP. After continuous treatment with 2 mM SNAP for 10 h, only 6.7% of the oocytes cleaved, and none developed beyond the 4-cell stage. Thirty-minute treatment repeated four times with 2 mM SNAP induced cleavage in 37.5% of the oocytes, 18.3% developed to the morula stage, and 6.7% reached the blastocyst stage. It is concluded that pulsatile treatment can improve parthenogenetic activation rate.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GG - Chov hospodářských zvířat

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Reproduction in Domestic Animals

ISSN

0936-6768

e-ISSN

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Svazek periodika

45

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

000277873000019

EID výsledku v databázi Scopus

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