Epigenetic Characteristics of Paternal Chromatin in Interspecies Zygotes
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F10%3A%230001310" target="_blank" >RIV/00027014:_____/10:#0001310 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Epigenetic Characteristics of Paternal Chromatin in Interspecies Zygotes
Popis výsledku v původním jazyce
Porcine sperm heads were injected into mouse ovulated and in vitro matured oocytes. Both pronuclei were formed after injection into mouse ovulated oocytes. Additional labelling against 5-MeC and some histone modification revealed similarity to those changes that are typical for mouse zygotes. After porcine sperm head injection into in vitro matured mouse oocytes, no paternal pronuclei were formed. In the other part of the study, mouse sperm head were injected into mouse in vitro matured oocytes. The labelling against 5-MeC showed less extensive demethylation than is typical for ovulated oocytes. Our observation suggest that porcine sperm heads are capable of undergoing active demethylation in vivo matured mouse oocytes and that in vitro matured mouse oocytes posses lower sperm remodeling capabilities.
Název v anglickém jazyce
Epigenetic Characteristics of Paternal Chromatin in Interspecies Zygotes
Popis výsledku anglicky
Porcine sperm heads were injected into mouse ovulated and in vitro matured oocytes. Both pronuclei were formed after injection into mouse ovulated oocytes. Additional labelling against 5-MeC and some histone modification revealed similarity to those changes that are typical for mouse zygotes. After porcine sperm head injection into in vitro matured mouse oocytes, no paternal pronuclei were formed. In the other part of the study, mouse sperm head were injected into mouse in vitro matured oocytes. The labelling against 5-MeC showed less extensive demethylation than is typical for ovulated oocytes. Our observation suggest that porcine sperm heads are capable of undergoing active demethylation in vivo matured mouse oocytes and that in vitro matured mouse oocytes posses lower sperm remodeling capabilities.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
ED - Fyziologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2010
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Reproduction and Development
ISSN
0916-8818
e-ISSN
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Svazek periodika
56
Číslo periodika v rámci svazku
6
Stát vydavatele periodika
JP - Japonsko
Počet stran výsledku
6
Strana od-do
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Kód UT WoS článku
000285859300006
EID výsledku v databázi Scopus
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