Interspecific ICSI for the Assessment of Sperm DNA Damage: Technology Report

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F21%3A00552881" target="_blank" >RIV/68378041:_____/21:00552881 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027014:_____/21:N0000038

Výsledek na webu

<a href="https://www.mdpi.com/2076-2615/11/5/1250" target="_blank" >https://www.mdpi.com/2076-2615/11/5/1250</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ani11051250" target="_blank" >10.3390/ani11051250</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Interspecific ICSI for the Assessment of Sperm DNA Damage: Technology Report

Popis výsledku v původním jazyce

The long-term storage of biological material (sperm, oocytes, embryos, etc.) is essential not only for animal breeding programs, human ART, and basic biology, but also for rescuing endangered animal species and other technologies. Whilst sperm storage is almost perfected for some species (bovine, human, mouse), many problems remain in others. In our contribution, we present a simple approach that can be used for the rapid evaluation of DNA damage level in sperm nuclei after freezing. This approach can be useful especially in those cases when the amount of frozen biological material is limited and permits much higher flexibility when modifying chosen preservation approaches.nnXenogenic mammalian sperm heads injected into mouse ovulated oocytes decondense and form pronuclei in which sperm DNA parameters can be evaluated. We suggest that this approach can be used for the assessment of sperm DNA damage level and the evaluation of how certain sperm treatments (freezing, lyophilization, etc.) influence the quality of spermatozoa.

Název v anglickém jazyce

Interspecific ICSI for the Assessment of Sperm DNA Damage: Technology Report

Popis výsledku anglicky

The long-term storage of biological material (sperm, oocytes, embryos, etc.) is essential not only for animal breeding programs, human ART, and basic biology, but also for rescuing endangered animal species and other technologies. Whilst sperm storage is almost perfected for some species (bovine, human, mouse), many problems remain in others. In our contribution, we present a simple approach that can be used for the rapid evaluation of DNA damage level in sperm nuclei after freezing. This approach can be useful especially in those cases when the amount of frozen biological material is limited and permits much higher flexibility when modifying chosen preservation approaches.nnXenogenic mammalian sperm heads injected into mouse ovulated oocytes decondense and form pronuclei in which sperm DNA parameters can be evaluated. We suggest that this approach can be used for the assessment of sperm DNA damage level and the evaluation of how certain sperm treatments (freezing, lyophilization, etc.) influence the quality of spermatozoa.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Animals

ISSN

2076-2615

e-ISSN

2076-2615

Svazek periodika

11

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

7

Strana od-do

1250

Kód UT WoS článku

000653360500001

EID výsledku v databázi Scopus

2-s2.0-85104581810