Use of Interspecific ICSI for the Assessment of Buck Sperm DNA Damage After Different Methods of Cryopreservation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F22%3AN0000290" target="_blank" >RIV/00027014:_____/22:N0000290 - isvavai.cz</a>

Výsledek na webu

<a href="https://vuzv.cz/_privat/22289.pdf" target="_blank" >https://vuzv.cz/_privat/22289.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Use of Interspecific ICSI for the Assessment of Buck Sperm DNA Damage After Different Methods of Cryopreservation

Popis výsledku v původním jazyce

The results obtained by measuring the level of γH2AX fluorescence intensity in the paternal pronuclei show that the values differ between the individual groups of semen. The lowest values of fluorescence intensity, and thus the lowest DNA damage, were found in freshly collected semen (11.93 ± 8.06). Variability was also demonstrated between the experimental groups of frozen semen. The influence of different methods of semen collection and its freezing and the influence of the composition of the freezing medium were confirmed. The highest values of fluorescence intensity were found in the freezing medium supplemented with egg yolk (39.23 ± 8.06). This method of assessing the quality of cryopreserved is a relatively simple and inexpensive method that can replace previous sperm quality assessment techniques with the advantage of replacing hard-to-reach oocytes of endangered species with available mouse oocytes

Název v anglickém jazyce

Use of Interspecific ICSI for the Assessment of Buck Sperm DNA Damage After Different Methods of Cryopreservation

Popis výsledku anglicky

The results obtained by measuring the level of γH2AX fluorescence intensity in the paternal pronuclei show that the values differ between the individual groups of semen. The lowest values of fluorescence intensity, and thus the lowest DNA damage, were found in freshly collected semen (11.93 ± 8.06). Variability was also demonstrated between the experimental groups of frozen semen. The influence of different methods of semen collection and its freezing and the influence of the composition of the freezing medium were confirmed. The highest values of fluorescence intensity were found in the freezing medium supplemented with egg yolk (39.23 ± 8.06). This method of assessing the quality of cryopreserved is a relatively simple and inexpensive method that can replace previous sperm quality assessment techniques with the advantage of replacing hard-to-reach oocytes of endangered species with available mouse oocytes

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10604 - Reproductive biology (medical aspects to be 3)

Projekt

<a href="/cs/project/QK1910156" target="_blank" >QK1910156: Nové postupy pro záchranu ohrožených populací hospodářských zvířat</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů