Optimization of Conditions of Prolonged Culture of Pig Granulosa Cells in Vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F11%3A%230001442" target="_blank" >RIV/00027014:_____/11:#0001442 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.vuzv.cz/sites/File/_privat/11078.pdf" target="_blank" >http://www.vuzv.cz/sites/File/_privat/11078.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Optimization of Conditions of Prolonged Culture of Pig Granulosa Cells in Vitro

Popis výsledku v původním jazyce

The object of the present study was to establish conditions (optimal serum concentration and growth factor supplement) for prolonged culture of pig granulosa cells (GC). GC were cultured in the presence of different fetal calf serum (FCS) concentrations(10% and 20%) and one of the following growth factors: leukemia inhibitory factor (LIF), basic fibroblast growth factor (bFGF) and stem cell factor (SCF). GC proliferation potential was measured by 3H-thymidine incorporation and telomerase activity whereas cell differentiation by relative transcript abundance of the aromatase gene. In the presence of 10% FCS GC proliferative potential was significantly higher (P<0.01) in comparison to control (medium only) after 9 days of culture. Basic fibroblast growth factor significantly increased (P<0.01) 3H-thymidine incorporation at all investigated time intervals/points, up to 18 days of culture. Telomerase activity was stimulated (P<0.01) by LIF, bFGF and SCF after 3, 6, 9, 12, 15 and 18 days o

Název v anglickém jazyce

Optimization of Conditions of Prolonged Culture of Pig Granulosa Cells in Vitro

Popis výsledku anglicky

The object of the present study was to establish conditions (optimal serum concentration and growth factor supplement) for prolonged culture of pig granulosa cells (GC). GC were cultured in the presence of different fetal calf serum (FCS) concentrations(10% and 20%) and one of the following growth factors: leukemia inhibitory factor (LIF), basic fibroblast growth factor (bFGF) and stem cell factor (SCF). GC proliferation potential was measured by 3H-thymidine incorporation and telomerase activity whereas cell differentiation by relative transcript abundance of the aromatase gene. In the presence of 10% FCS GC proliferative potential was significantly higher (P<0.01) in comparison to control (medium only) after 9 days of culture. Basic fibroblast growth factor significantly increased (P<0.01) 3H-thymidine incorporation at all investigated time intervals/points, up to 18 days of culture. Telomerase activity was stimulated (P<0.01) by LIF, bFGF and SCF after 3, 6, 9, 12, 15 and 18 days o

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Annals of Animal Science 

ISSN

1642-3402

e-ISSN

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Svazek periodika

11

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

10

Strana od-do

383-392

Kód UT WoS článku

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EID výsledku v databázi Scopus

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