Quantitative analysis of oestrogen receptor alpha and beta gene expression during prolonged culture of pig granulosa cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F12%3A%230001661" target="_blank" >RIV/00027014:_____/12:#0001661 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.vuzv.cz/sites/File/_privat/12104.pdf" target="_blank" >http://www.vuzv.cz/sites/File/_privat/12104.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Quantitative analysis of oestrogen receptor alpha and beta gene expression during prolonged culture of pig granulosa cells

Popis výsledku v původním jazyce

The aim of the present work was to determine the relative transcript abundance of the oestrogen receptor alpha (ER alpha) and beta (ER beta) gene in long-term cultures (up to 18 days) of pig granulosa cells (GC) obtained from small (1-2 mm; SF-GC) and large (5-7 mm; LF-GC) follicles. GC were cultured in the presence of 10% foetal calf serum (FCS) and one of the following growth factors: leukaemia inhibitory factor (LIF), basic fibroblast growth factor (bFGF), or stem cell factor (SCF). The relative transcript abundance of the investigated genes was measured by real time PCR (RT-PCR). In comparison with the control (medium only), LIF significantly increased (P<0.05-0.01) ER alpha gene expression in SF-GC after 3, 15 and 18 days of culture and after 12 days in LF-GC. The ER beta mRNA level in SF-GC was significantly decreased (P<0.01) by all of the investigated factors after 3, 6, 9 and 12 days of incubation. In conclusion, expression of both types of oestrogen receptors during prolonged

Název v anglickém jazyce

Quantitative analysis of oestrogen receptor alpha and beta gene expression during prolonged culture of pig granulosa cells

Popis výsledku anglicky

The aim of the present work was to determine the relative transcript abundance of the oestrogen receptor alpha (ER alpha) and beta (ER beta) gene in long-term cultures (up to 18 days) of pig granulosa cells (GC) obtained from small (1-2 mm; SF-GC) and large (5-7 mm; LF-GC) follicles. GC were cultured in the presence of 10% foetal calf serum (FCS) and one of the following growth factors: leukaemia inhibitory factor (LIF), basic fibroblast growth factor (bFGF), or stem cell factor (SCF). The relative transcript abundance of the investigated genes was measured by real time PCR (RT-PCR). In comparison with the control (medium only), LIF significantly increased (P<0.05-0.01) ER alpha gene expression in SF-GC after 3, 15 and 18 days of culture and after 12 days in LF-GC. The ER beta mRNA level in SF-GC was significantly decreased (P<0.01) by all of the investigated factors after 3, 6, 9 and 12 days of incubation. In conclusion, expression of both types of oestrogen receptors during prolonged

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Animal and Feed Sciences

ISSN

1230-1388

e-ISSN

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Svazek periodika

21

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

11

Strana od-do

313-323

Kód UT WoS článku

000306726400008

EID výsledku v databázi Scopus

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