Effect of antioestrogens on proliferation, differentiation and telomerase activity of pig granulosa cells in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F10%3A%230001285" target="_blank" >RIV/00027014:_____/10:#0001285 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of antioestrogens on proliferation, differentiation and telomerase activity of pig granulosa cells in vitro

Popis výsledku v původním jazyce

The aim of the work was to study the effect of oestrogen receptor (ER) inhibitors (ICI 164.384; ICI and cyclofenil; CF) on proliferation, oestradiol synthesis, aromatase expression and telomerase activity (TA) of pig granulosa cells (GC). Antioestrogensapplied individually and in a combination significantly (P<0.01) decreased proliferative potential of LF-GC. Antioestrogens applied in a combination increased (P<0.05) 17-? oestradiol synthesis in small and large follicle GC. Antioestrogens applied individually and in a combination caused an increase (P<0.01) of aromatase gene expression in FSH-stimulated conditions as well as in basic conditions. Moreover, antioestrogens increased (P<0.05) TA in basic and in FSH-stimulated conditions. The results indicate the involvement of ER ? and ? in the control of proliferation, differentiation and telomerase activity in pig GC. Furthermore, TA does not have to be linked only to pig GC proliferation but may also be involved in the differentiation

Název v anglickém jazyce

Effect of antioestrogens on proliferation, differentiation and telomerase activity of pig granulosa cells in vitro

Popis výsledku anglicky

The aim of the work was to study the effect of oestrogen receptor (ER) inhibitors (ICI 164.384; ICI and cyclofenil; CF) on proliferation, oestradiol synthesis, aromatase expression and telomerase activity (TA) of pig granulosa cells (GC). Antioestrogensapplied individually and in a combination significantly (P<0.01) decreased proliferative potential of LF-GC. Antioestrogens applied in a combination increased (P<0.05) 17-? oestradiol synthesis in small and large follicle GC. Antioestrogens applied individually and in a combination caused an increase (P<0.01) of aromatase gene expression in FSH-stimulated conditions as well as in basic conditions. Moreover, antioestrogens increased (P<0.05) TA in basic and in FSH-stimulated conditions. The results indicate the involvement of ER ? and ? in the control of proliferation, differentiation and telomerase activity in pig GC. Furthermore, TA does not have to be linked only to pig GC proliferation but may also be involved in the differentiation

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA523%2F05%2F2062" target="_blank" >GA523/05/2062: Vývojový potenciál a molekulární diferenciace folikulárních granulózních buněk prasete in vitro</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Animal and Feed Sciences

ISSN

1230-1388

e-ISSN

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Svazek periodika

19

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

13

Strana od-do

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Kód UT WoS článku

000286028700007

EID výsledku v databázi Scopus

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