Bifidobacteria, Lactobacilli, and Short Chain Fatty Acids of Vegetarians and Omnivores

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F17%3AN0000073" target="_blank" >RIV/00027014:_____/17:N0000073 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/17:73661

Výsledek na webu

<a href="https://vuzv.cz/_privat/17063.pdf" target="_blank" >https://vuzv.cz/_privat/17063.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1515/sab-2017-0007" target="_blank" >10.1515/sab-2017-0007</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Bifidobacteria, Lactobacilli, and Short Chain Fatty Acids of Vegetarians and Omnivores

Popis výsledku v původním jazyce

The intestinal microbiota represents the largest and the most complex microbial community inhabiting the human body. Bifidobacteria and lactobacilli represent important commensal bacteria with the ability to utilize complex carbohydrates. The main fermentation products from the breakdown of complex dietary carbohydrates are short chain fatty acids (SCFAs). We examined faecal samples of vegetarians (n = 10) and conventional omnivores (n = 10) to evaluate the counts and occurrence of cultivable bacteria, especially bifidobacteria and lactobacilli, using cultivation on selective media, and matrix-assisted laser desorption/ionization time-of-flight. Moreover, concentrations and molar proportion of SCFAs in faecal samples were measured. Total counts of Gram-negative anaerobic bacteria were significantly lower (P < 0.05) in vegetarian faecal samples, while others (total anaerobic bacteria, Bifidobacterium spp., Lactobacillus spp., Escherichia coli, and presumptive coliforms) were not. Neither total concentrations nor molar proportions of SCFAs in faecal samples differed (P > 0.05) between the diet groups. In total, six Bifidobacterium spp. and thirteen Lactobacillus spp. were detected via culture-dependent methods. Bifidobacteria counts and species composition in faecal samples of both groups were found to be relatively similar, regardless of the diet. Lactobacillus species varied more by individual diet.

Název v anglickém jazyce

Bifidobacteria, Lactobacilli, and Short Chain Fatty Acids of Vegetarians and Omnivores

Popis výsledku anglicky

The intestinal microbiota represents the largest and the most complex microbial community inhabiting the human body. Bifidobacteria and lactobacilli represent important commensal bacteria with the ability to utilize complex carbohydrates. The main fermentation products from the breakdown of complex dietary carbohydrates are short chain fatty acids (SCFAs). We examined faecal samples of vegetarians (n = 10) and conventional omnivores (n = 10) to evaluate the counts and occurrence of cultivable bacteria, especially bifidobacteria and lactobacilli, using cultivation on selective media, and matrix-assisted laser desorption/ionization time-of-flight. Moreover, concentrations and molar proportion of SCFAs in faecal samples were measured. Total counts of Gram-negative anaerobic bacteria were significantly lower (P < 0.05) in vegetarian faecal samples, while others (total anaerobic bacteria, Bifidobacterium spp., Lactobacillus spp., Escherichia coli, and presumptive coliforms) were not. Neither total concentrations nor molar proportions of SCFAs in faecal samples differed (P > 0.05) between the diet groups. In total, six Bifidobacterium spp. and thirteen Lactobacillus spp. were detected via culture-dependent methods. Bifidobacteria counts and species composition in faecal samples of both groups were found to be relatively similar, regardless of the diet. Lactobacillus species varied more by individual diet.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientia Agriculturae Bohemica

ISSN

1211-3174

e-ISSN

—

Svazek periodika

48

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

47-54

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85017500500