Detekce fekálních bakterií FISH metodou.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F06%3A17258" target="_blank" >RIV/60460709:41210/06:17258 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Detection of faecal bacteria by FISH method.

Popis výsledku v původním jazyce

Cultivation methods for the quantitative detection of the faecal bacteria are inaccurate and time consuming, also not all bacteria are cultivatable and results obtained may be incorrect. Hence, the aim of our study was to evaluate FISH method for the quantitative detection of different bacterial group in faecal samples of infants, calves, and lambs during the milk-feeding period. Faecal samples from 91 infants, 20 calves, and 18 lambs were tested. A specific fluorescence in situ hybridisation (FISH) kitfor Bifidobacterium spp., Clostridium butyricum group, Escherichia coli, and Lactobacillus spp. were used for the quantitative detection of bacteria compared to cultivation methods. Sixty-nine infants possessed high numbers of bifidobacteria in their faeces. Cultivation counts were nonsignificantly lower (9.91 ? 0.67) than counts determinated by FISH (9.92 ? 0.55). Twenty-two infants did not contain detectable bifidobacteria. These infants possessed as a major group of their faecal f

Název v anglickém jazyce

Detection of faecal bacteria by FISH method.

Popis výsledku anglicky

Cultivation methods for the quantitative detection of the faecal bacteria are inaccurate and time consuming, also not all bacteria are cultivatable and results obtained may be incorrect. Hence, the aim of our study was to evaluate FISH method for the quantitative detection of different bacterial group in faecal samples of infants, calves, and lambs during the milk-feeding period. Faecal samples from 91 infants, 20 calves, and 18 lambs were tested. A specific fluorescence in situ hybridisation (FISH) kitfor Bifidobacterium spp., Clostridium butyricum group, Escherichia coli, and Lactobacillus spp. were used for the quantitative detection of bacteria compared to cultivation methods. Sixty-nine infants possessed high numbers of bifidobacteria in their faeces. Cultivation counts were nonsignificantly lower (9.91 ? 0.67) than counts determinated by FISH (9.92 ? 0.55). Twenty-two infants did not contain detectable bifidobacteria. These infants possessed as a major group of their faecal f

Druh

D - Stať ve sborníku

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GP523%2F05%2FP117" target="_blank" >GP523/05/P117: Výskyt a úloha bifidobakterií u mláďat přežvýkavců v období mléčné výživy</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Book of Abstracts Genetics of industrial microorganisms.

ISBN

80-239-7323-1

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

0-0

Název nakladatele

Institute of Microbiologzy ASCR

Místo vydání

Prague

Místo konání akce

Prague

Datum konání akce

24. 6. 2006

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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