Nucleologenesis and nucleolotransfer in mammalian oocytes: A review

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F17%3AN0000193" target="_blank" >RIV/00027014:_____/17:N0000193 - isvavai.cz</a>

Výsledek na webu

<a href="https://vuzv.cz/_privat/17195.pdf" target="_blank" >https://vuzv.cz/_privat/17195.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1515/macvetrev-2017-0023" target="_blank" >10.1515/macvetrev-2017-0023</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Nucleologenesis and nucleolotransfer in mammalian oocytes: A review

Popis výsledku v původním jazyce

An effort to improve development potential of early embryos is one of the main goals of biotechnology in the area of reproductive biology with application in veterinary or human medicine. Recent observations of the function of nucleolus or rather its forms before, during and after the fertilisation or parthenogenetic activation show the key role(s) of nucleolus in the processes of early genome activation. The nucleolus is a subnuclear structure (organelle) mainly involved in regulation of transcription and translation. This organelle has been characterized in detail by immunofluorescence, cell transfection and proteomics. This data was, however, mostly obtained in nucleoli of differentiated eukaryotic cells. Much less is known about the nucleolar structural changes and related functional processes in growing and fully grown mammalian oocytes, zygotes and early cleavage stage embryos, especially in the context of embryonic genome activation. It has been shown, that nucleoli in mammalian oocytes and early embryos have several forms and functions, which vary during the oocyte growth and embryonic development. Certain functions have not been fully described or explained, yet. The method of enucleolation, which allows to remove nucleoli from the oocytes or to exchange nucleoli between oocytes or zygotes, together with their proteomic and structural analyses brought new information about functions of nucleoli in oocytes and early cleavage-stage embryos and allowed to explain some new key roles of nucleoli during oocyte maturation and early embryonic development.

Název v anglickém jazyce

Nucleologenesis and nucleolotransfer in mammalian oocytes: A review

Popis výsledku anglicky

An effort to improve development potential of early embryos is one of the main goals of biotechnology in the area of reproductive biology with application in veterinary or human medicine. Recent observations of the function of nucleolus or rather its forms before, during and after the fertilisation or parthenogenetic activation show the key role(s) of nucleolus in the processes of early genome activation. The nucleolus is a subnuclear structure (organelle) mainly involved in regulation of transcription and translation. This organelle has been characterized in detail by immunofluorescence, cell transfection and proteomics. This data was, however, mostly obtained in nucleoli of differentiated eukaryotic cells. Much less is known about the nucleolar structural changes and related functional processes in growing and fully grown mammalian oocytes, zygotes and early cleavage stage embryos, especially in the context of embryonic genome activation. It has been shown, that nucleoli in mammalian oocytes and early embryos have several forms and functions, which vary during the oocyte growth and embryonic development. Certain functions have not been fully described or explained, yet. The method of enucleolation, which allows to remove nucleoli from the oocytes or to exchange nucleoli between oocytes or zygotes, together with their proteomic and structural analyses brought new information about functions of nucleoli in oocytes and early cleavage-stage embryos and allowed to explain some new key roles of nucleoli during oocyte maturation and early embryonic development.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

10604 - Reproductive biology (medical aspects to be 3)

Projekt

<a href="/cs/project/GA17-08605S" target="_blank" >GA17-08605S: Jaderné a cytoplasmatické determinanty zodpovědné za regulaci zrání oocytů a vývoj embrya</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Macedonian Veterinary Review

ISSN

1409-7621

e-ISSN

—

Svazek periodika

40

Číslo periodika v rámci svazku

248

Stát vydavatele periodika

MK - Republika Severní Makedonie

Počet stran výsledku

8

Strana od-do

117-124

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85031778569