Post-harvest motility and morphological changes of spermatozoa following caudal epididymal recovery in farmed common eland (Tragelaphus oryx) bulls

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F25%3A10006292" target="_blank" >RIV/00027014:_____/25:10006292 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41340/25:103758 RIV/62157124:16170/25:43882222

Výsledek na webu

<a href="https://vuzv.cz/_privat/25032.pdf" target="_blank" >https://vuzv.cz/_privat/25032.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.repbio.2025.100997" target="_blank" >10.1016/j.repbio.2025.100997</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Post-harvest motility and morphological changes of spermatozoa following caudal epididymal recovery in farmed common eland (Tragelaphus oryx) bulls

Popis výsledku v původním jazyce

The caudal epididymal recovery of spermatozoa has been utilised in game animal species to preserve genetically superior material after trophy hunting or slaughter, due to the difficulties of handling wild animals. Furthermore, the potential application of assisted reproductive techniques using harvested spermatozoa may contribute towards maintaining genetic diversity in isolated captive populations internationally. However, this technique requires clear and ideally simple protocols for the collection and handling of gametes after the death of the male animals, as the point of harvest is usually remote in game ranching. Therefore, this research aimed to investigate the effects of time after harvesting sperm under basic field conditions from the caudal epididymides of farmed common eland bulls in terms of their sperm motility and morphological changes. The relationship among these sperm quality parameters was also explored. Six bulls (2-2.5 years old; 203 +/- 20 kg) were slaughtered, and their epididymal sperm were harvested and assessed for sperm motility and kinematics at minutes 0, 35, 70, 100, together with sperm viability, sperm head morphometry, and morphology at minutes 0, 20, 40, 60, 80 using CASA. Sperm quality sharply declined after 35 minutes of slaughter, but no effects were seen in sperm head morphometry. This study brings the first information regarding the quality of sperm retrieval from the cauda epididymides and sperm quality under physiological conditions in a uniform age group of eland. Future work should consider the effect of animal age and other individual animal traits, as well as the use of various media and extenders.

Název v anglickém jazyce

Post-harvest motility and morphological changes of spermatozoa following caudal epididymal recovery in farmed common eland (Tragelaphus oryx) bulls

Popis výsledku anglicky

The caudal epididymal recovery of spermatozoa has been utilised in game animal species to preserve genetically superior material after trophy hunting or slaughter, due to the difficulties of handling wild animals. Furthermore, the potential application of assisted reproductive techniques using harvested spermatozoa may contribute towards maintaining genetic diversity in isolated captive populations internationally. However, this technique requires clear and ideally simple protocols for the collection and handling of gametes after the death of the male animals, as the point of harvest is usually remote in game ranching. Therefore, this research aimed to investigate the effects of time after harvesting sperm under basic field conditions from the caudal epididymides of farmed common eland bulls in terms of their sperm motility and morphological changes. The relationship among these sperm quality parameters was also explored. Six bulls (2-2.5 years old; 203 +/- 20 kg) were slaughtered, and their epididymal sperm were harvested and assessed for sperm motility and kinematics at minutes 0, 35, 70, 100, together with sperm viability, sperm head morphometry, and morphology at minutes 0, 20, 40, 60, 80 using CASA. Sperm quality sharply declined after 35 minutes of slaughter, but no effects were seen in sperm head morphometry. This study brings the first information regarding the quality of sperm retrieval from the cauda epididymides and sperm quality under physiological conditions in a uniform age group of eland. Future work should consider the effect of animal age and other individual animal traits, as well as the use of various media and extenders.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10604 - Reproductive biology (medical aspects to be 3)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Reproductive Biology

ISSN

1642-431X

e-ISSN

—

Svazek periodika

25

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

"Article Number 100997"

Kód UT WoS článku

001431969300001

EID výsledku v databázi Scopus

2-s2.0-85218076684