Simple and cheap isolation and purification of alkali-soluble ß-glucans from Pleurotus spp. and Lentinus edodes mushrooms (Article: Proceedings of the 5th International Mushroom Conference. 5th ? 8th September 2009. Nantong, China)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027022%3A_____%2F09%3A00000125" target="_blank" >RIV/00027022:_____/09:00000125 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Simple and cheap isolation and purification of alkali-soluble ß-glucans from Pleurotus spp. and Lentinus edodes mushrooms (Article: Proceedings of the 5th International Mushroom Conference. 5th ? 8th September 2009. Nantong, China)

Popis výsledku v původním jazyce

We developed a new, simple, cheap and ecological method of extraction of alkali-soluble ß-glucans from Pleurotus spp. and Lentinula edodes mushrooms. In the first step, mushroom biomass is hydrolyzed in a diluted aqueous solution of hydrochloric acid under heating. A significant part of mushroom biopolymers - proteins, acid-soluble polysaccharides, nucleic acids, and lipids - is hydrolyzed and solubilized under the treatment. The product containing the alkali-insoluble ß-glucans was obtained in form ofa segregated hydrogel upper layer of sediment after centrifugation of the acid-hydrolyzed mushroom suspension. Chitin and insoluble skeletal ß-glucans can be separated from the remaining sediment in a subsequent step by alkali hydrolysis or another method described in literature.

Název v anglickém jazyce

Simple and cheap isolation and purification of alkali-soluble ß-glucans from Pleurotus spp. and Lentinus edodes mushrooms (Article: Proceedings of the 5th International Mushroom Conference. 5th ? 8th September 2009. Nantong, China)

Popis výsledku anglicky

We developed a new, simple, cheap and ecological method of extraction of alkali-soluble ß-glucans from Pleurotus spp. and Lentinula edodes mushrooms. In the first step, mushroom biomass is hydrolyzed in a diluted aqueous solution of hydrochloric acid under heating. A significant part of mushroom biopolymers - proteins, acid-soluble polysaccharides, nucleic acids, and lipids - is hydrolyzed and solubilized under the treatment. The product containing the alkali-insoluble ß-glucans was obtained in form ofa segregated hydrogel upper layer of sediment after centrifugation of the acid-hydrolyzed mushroom suspension. Chitin and insoluble skeletal ß-glucans can be separated from the remaining sediment in a subsequent step by alkali hydrolysis or another method described in literature.

Druh

O - Ostatní výsledky

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/2B06173" target="_blank" >2B06173: Materiály a produkty s vysokou přidanou hodnotou ze zemědělských a potravinářských odpadů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů